Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1969 Apr;17(4):516–519. doi: 10.1128/am.17.4.516-519.1969

Biological Activity of Aflatoxin B2a

E B Lillehoj a, A Ciegler a
PMCID: PMC377733  PMID: 5772392

Abstract

The toxicity of alfatoxin B2a (hydroxydihydro-aflatoxin B1) was studied in several biological systems. Aflatoxin B2a is the monohydroxylated derivative obtained from addition of water to the double bond of the terminal furan of B1. Examination of the sensitivity of a group of microorganisms to B2a demonstrated that the inhibitory spectrum was similar to aflatoxin B1. However, the toxicity of B2a was markedly lower than B1, as measured by the initiation of bile duct hyperplasia in ducklings. Binding of aflatoxin to deoxyribonucleic acid (DNA) was determined by measuring the hypochromicity produced by the nucleic acid at 363 nm and the capacity of increasing amounts of DNA to quench the fluorescence of the toxin was also used as a measure of the binding of toxin to nucleic acid. These tests showed that the DNA-binding capacity of B2a was lower than B1.

Full text

PDF
516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black H. S., Jirgensons B. Interactions of aflatoxin with histones and DNA. Plant Physiol. 1967 May;42(5):731–735. doi: 10.1104/pp.42.5.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burmeister H. R., Hesseltine C. W. Survey of the sensitivity of microorganisms to aflatoxin. Appl Microbiol. 1966 May;14(3):403–404. doi: 10.1128/am.14.3.403-404.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARNAGHAN R. B., HARTLEY R. D., O'KELLY J. TOXICITY AND FLUORESCENCE PROPERTIES OF THE AFLATOXINS. Nature. 1963 Dec 14;200:1101–1101. doi: 10.1038/2001101a0. [DOI] [PubMed] [Google Scholar]
  4. Ciegler A., Lillehoj E. B., Peterson R. E., Hall H. H. Microbial detoxification of aflatoxin. Appl Microbiol. 1966 Nov;14(6):934–939. doi: 10.1128/am.14.6.934-939.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ciegler A., Peterson R. E. Aflatoxin detoxification: hydroxydihydro-aflatoxin B. Appl Microbiol. 1968 Apr;16(4):665–666. doi: 10.1128/am.16.4.665-666.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clifford J. I., Rees K. R. Aflatoxin: a site of action in the rat liver cell. Nature. 1966 Jan 15;209(5020):312–313. doi: 10.1038/209312a0. [DOI] [PubMed] [Google Scholar]
  7. Clifford J. I., Rees K. R. The interaction of aflatoxins with purines and purine nucleosides. Biochem J. 1967 May;103(2):467–471. doi: 10.1042/bj1030467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dutton M. F., Heathcote J. G. The structure, biochemical properties and origin of the aflatoxins B2a and G2a. Chem Ind. 1968 Mar 30;13:418–421. [PubMed] [Google Scholar]
  9. Finkelstein T., Weinstein I. B. Proflavine binding to transfer ribonucleic acid, synthetic ribonucleic acids, and deoxyribonucleic acid. J Biol Chem. 1967 Sep 10;242(17):3763–3768. [PubMed] [Google Scholar]
  10. HAYNES W. C., WICKERHAM L. J., HESSELTINE C. W. Maintenance of cultures of industrially important microorganisms. Appl Microbiol. 1955 Nov;3(6):361–368. doi: 10.1128/am.3.6.361-368.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holzapfel C. W., Steyn P. S., Purchase I. F. Isolation and structure of aflatoxins M1 and M2. Tetrahedron Lett. 1966 Jun;25:2799–2803. doi: 10.1016/s0040-4039(01)99863-6. [DOI] [PubMed] [Google Scholar]
  12. Purchase I. F. Acute toxicity of aflatoxins M1 and M2 in one-day-old ducklings. Food Cosmet Toxicol. 1967 Aug;5(3):339–342. doi: 10.1016/s0015-6264(67)83060-8. [DOI] [PubMed] [Google Scholar]
  13. Shotwell O. L., Hesseltine C. W., Stubblefield R. D., Sorenson W. G. Production of aflatoxin on rice. Appl Microbiol. 1966 May;14(3):425–428. doi: 10.1128/am.14.3.425-428.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sporn M. B., Dingman C. W., Phelps H. L., Wogan G. N. Aflatoxin B1: binding to DNA in vitro and alteration of RNA metabolism in vivo. Science. 1966 Mar 25;151(3717):1539–1541. doi: 10.1126/science.151.3717.1539. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES