Abstract
An improved method for increasing cell yield from animal tissues is described. The current technique differs from the classical, primarily in that the tissues were perfused with trypsin in situ and cell dispersal was completed by trypsinization either at 4 or 37 C but without prior mincing of the tissue. Increased yields of 75% were obtained. Mechanical damage to tissue by mincing was presumed to be the greatest single source of injury to cells. The adoption of the trypsin perfusion technique reduced the number of animals needed to prepare an equivalent quantity of tissue culture.
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- BISHOP L. W., SMITH M. K., BEALE A. J. An apparatus for producing trypsinized monkey kidney cell suspensions. Virology. 1960 Feb;10:280–283. doi: 10.1016/0042-6822(60)90048-9. [DOI] [PubMed] [Google Scholar]
- Chagnon A., Pavilanis V. Freezing and storing of monkey and rabbit kidney cells for tissue cultures. Cryobiology. 1966 Sep-Oct;3(2):81–84. doi: 10.1016/s0011-2240(66)80004-4. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAPPAPORT C. Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions. Bull World Health Organ. 1956;14(1):147–166. [PMC free article] [PubMed] [Google Scholar]
- YOUNGNER J. S. Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc Soc Exp Biol Med. 1954 Feb;85(2):202–205. doi: 10.3181/00379727-85-20830. [DOI] [PubMed] [Google Scholar]