Abstract
A group of 2,081 sputum specimens were planted on Direct Cord Reading Agar. A total of 330 (16%) of these specimens produced positive cultures, 312 strains of Mycobacterium tuberculosis and 18 strains of atypical mycobacteria. The strains of M. tuberculosis showed visible cords on plates in 283 (90.8%) of the isolates and no atypical mycobacteria showed cording. The specimens from the newly admitted patients yielded, in 12 days, 59% of their total positives; 97.6% of these strains, identified as M. tuberculosis with the standard methods recommended by the National Communicable Diseases Center, exhibited cording visible directly on the agar. It is suggested that, for practical purposes, a colony exhibiting cording could be tentatively reported as “M. tuberculosis to be confirmed.”
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DARZINS E., FAHR G. Cord-forming property, lethality and pathogenicity of Mycobacteria. Dis Chest. 1956 Dec;30(6):642–648. doi: 10.1378/chest.30.6.642. [DOI] [PubMed] [Google Scholar]
- Kestle D. G., Abbott V. D., Kubica G. P. Differential identification of mycobacteria. II. Subgroups of Groups II and 3 (Runyon) with different clinical significance. Am Rev Respir Dis. 1967 Jun;95(6):1041–1052. doi: 10.1164/arrd.1967.95.6.1041. [DOI] [PubMed] [Google Scholar]
- Lorian V. Comparison of mycobacterial cultures on agar and Lowenstein medium. Appl Microbiol. 1967 Sep;15(5):1202–1205. doi: 10.1128/am.15.5.1202-1205.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lorian V. Differentiation of Mycobacterium tuberculosis and Runyon Group 3 "V" strains on direct cord-reading agar. Am Rev Respir Dis. 1968 Jun;97(6):1133–1135. doi: 10.1164/arrd.1968.97.6P1.1133. [DOI] [PubMed] [Google Scholar]
- Lorian V. Direct cord reading medium for isolation of mycobacteria. Appl Microbiol. 1966 Jul;14(4):603–607. doi: 10.1128/am.14.4.603-607.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TARSHIS M. S. Thioglycollate, peroxidase, neutral red, serpentine cord, and niacin tests for group differentiation of M. tuberculosis, anonymous (atypical) acidfast bacilli, and saprophytic mycobacteria. Dis Chest. 1960 Oct;38:413–416. doi: 10.1378/chest.38.4.413. [DOI] [PubMed] [Google Scholar]