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. 1969 Apr;17(4):589–595. doi: 10.1128/am.17.4.589-595.1969

Markers Differentiating Type 3 Parainfluenza Virus Grown at 25 and 37 C

Paul H Frickey a,1
PMCID: PMC377748  PMID: 4306261

Abstract

Serial passage of the 64-2389 strain of type 3 parainfluenza virus in cercopithecus monkey kidney tissue cultures at low temperatures resulted in the selection of a variant which had a higher efficiency of plaque formation at 25 C than the parent line grown at 37 C. The cold variant, unlike the parent strain, plaqued readily at 25 C, and at 37 C it produced significantly larger plaques. Virus titers of the cold variant in hamster lungs were significantly lower and this was probably caused by the stimulation of interferon by the cold variant during the early phase of the infection. The cold variant, like the virus grown at 37 C, also induced the synthesis of interferon late in the infection. Hamsters responded to the intranasal inoculation of each virus line by the development of hemagglutinating-inhibiting antibodies in the sera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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