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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Biochemistry. 2013 Aug 14;52(34):5941–5951. doi: 10.1021/bi400541v

Figure 3.

Figure 3

Resonance Raman spectra of the νFe-CO and νCO modes of 12CO and 13CO bound H93G, H93G (THT) and H93G (CPSH). The low frequency Fe-CO stretching and bending region is in the left panels. The high frequency CO stretching is in the right panels and the 13CO – 12CO difference is shown in blue. Excitation wavelength is 413 nm and laser power at the sample is 11mw. The sample was in a cell spinning at 6000 rpm. Dashed and dotted lines indicate νFeCO modes of H93G(His)CO and H93G(H2O)CO respectively.