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Applied Microbiology logoLink to Applied Microbiology
. 1969 Apr;17(4):619–622. doi: 10.1128/am.17.4.619-622.1969

Minicultures of Mammalian Cells in a New Plastic Plate

David A Fuccillo 1,2,3, Louis W Catalano Jr 1,2,3, Flora L Moder 1,2,3, David A Debus 1,2,3,1, John L Sever 1,2,3
PMCID: PMC377754  PMID: 4306263

Abstract

A new disposable micro tissue culture plate was developed and tested for use in virological procedures. Miniature mammalian cell cultures (minicultures) were grown in these plates. Each plate contained 96 circular cultures in flat wells (7 mm in diameter). Replicate titrations of a number of viruses were performed in various tissues. Excellent reproducibility was demonstrated. Mean infectivity titers determined by miniculture methods were generally within 0.6 log10/ml of macro tube titrations. Standard tissue culture assay techniques such as hemadsorption, interference titration, and microneutralization were easily carried out with this method and were very reproducible. Development of this noncytotoxic disposable micro tissue culture plate now permits the routine performance of rapid, reliable, and reproducible tissue culture tests at a very significant reduction in cost and labor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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