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. 1969 Apr;17(4):636–638. doi: 10.1128/am.17.4.636-638.1969

Concentration and Purification of Herpesviruses (Simplex, Cytomegalo, and EB) in a Zonal Ultracentrifuge

Robert M McCombs 1
PMCID: PMC377759  PMID: 4306264

Abstract

By using the BXIV zonal ultracentrifuge rotor, it was possible to efficiently concentrate and partially purify members of the herpesvirus group by pelleting the virus onto a double sucrose cushion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson N. G., Waters D. A., Fisher W. D., Cline G. B., Nunley C. E., Elrod L. H., Rankin C. T., Jr Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors. Anal Biochem. 1967 Nov;21(2):235–252. doi: 10.1016/0003-2697(67)90185-6. [DOI] [PubMed] [Google Scholar]
  2. Benyesh-Melnick M., Phillips C. F., Lewis R. T., Seidel E. H. Studies on acute leukemia and infectious mononucleosis of childhood. IV. Continuous propagation of lymphoblastoid cells from spontaneously transformed bone marrow cultures. J Natl Cancer Inst. 1968 Jan;40(1):123–134. [PubMed] [Google Scholar]
  3. Benyesh-Melnick M., Probstmeyer F., McCombs R., Brunschwig J. P., Vonka V. Correlation between infectivity and physical virus particles in human cytomegalovirus. J Bacteriol. 1966 Nov;92(5):1555–1561. doi: 10.1128/jb.92.5.1555-1561.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berman A. S. Theory of centrifugation: miscellaneous studies. Natl Cancer Inst Monogr. 1966 Jun;21:41–76. [PubMed] [Google Scholar]
  5. Fox S. M., Birnie G. D., Harvey D. R., Martin E. M., Sonnabend J. A. The use of batch-type zonal ultracentrifuge rotors for the isolation and purification of viruses. J Gen Virol. 1968 May;2(3):455–459. doi: 10.1099/0022-1317-2-3-455. [DOI] [PubMed] [Google Scholar]
  6. McCombs R. M., Melnick M. B., Brunschwig J. P. Biophysical studies of vesicular stomatitis virus. J Bacteriol. 1966 Feb;91(2):803–812. doi: 10.1128/jb.91.2.803-812.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Vonka V., Benyesh-Melnick M. Interactions of human cytomegalovirus with human fibroblasts. J Bacteriol. 1966 Jan;91(1):213–220. doi: 10.1128/jb.91.1.213-220.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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