Abstract
A direct fluorometric test for the rapid detection of group A streptococci from throat swabs was compared with the microscopic fluorescent-antibody test. Formalinized throat swab cultures (490) were examined by the two methods, and the results agreed on 84% of the specimens. In another comparison, 15-hr broth cultures of 103 freshly taken throat swabs were tested by both methods. Of the specimens tested, 101 (98%) were either positive or negative by both methods. In all cases, the latter results correlated with the demonstration of presence or absence of group A streptococci in the specimens by cultural isolation and precipitin grouping tests. It may be feasible to use the direct fluorometric test in a diagnostic laboratory as described or possibly to adapt it for automatic processing of throat swab cultures.
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Selected References
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- Hochberg H. M., Cooper J. K., Redys J. J., Caceres C. A. Identification of fluorescent-antibody labeled group A streptococci by fluorometry. Appl Microbiol. 1966 May;14(3):386–390. doi: 10.1128/am.14.3.386-390.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCKINNEY R. M., SPILLANE J. T., PEARCE G. W. FLUORESCEIN DIACETATE AS A REFERENCE COLOR STANDARD IN FLUORESCENT ANTIBODY STUDIES. Anal Biochem. 1964 Dec;9:474–476. doi: 10.1016/0003-2697(64)90208-8. [DOI] [PubMed] [Google Scholar]
- Moody M. D., Siegel A. C., Pittman B., Winter C. C. Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs. Am J Public Health Nations Health. 1963 Jul;53(7):1083–1092. doi: 10.2105/ajph.53.7.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moody M. D., Webb C. D., Jr Identification of group A streptococci by direct fluorometry. Appl Microbiol. 1969 Apr;17(4):627–633. doi: 10.1128/am.17.4.627-633.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]