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. 1969 May;17(5):685–689. doi: 10.1128/am.17.5.685-689.1969

Enhanced Recovery of Airborne T3 Coliphage and Pasteurella pestis Bacteriophage by Means of a Presampling Humidification Technique

M T Hatch 1,2, J C Warren 1,2
PMCID: PMC377780  PMID: 4891719

Abstract

This paper reports a series of experiments in which two methods of collecting airborne bacteriophage particles were compared. A standard aerosol sampler, the AGI-30, was evaluated for its competence in measuring the content of bacteriophage aerosols. It was used alone or with a prewetting or humidification device (humidifier bulb) to recover T3 coliphage and Pasteurella pestis bacteriophage particles from aerosols maintained at 21 C and varied relative humidity. Collection of bacteriophage particles via the humidifier bulb altered both the initial recovery level and the apparent biological decay. Sampling airborne bacteriophage particles by the AGI-30 alone yielded data that apparently underestimated the maximal number of potentially viable particles within the aerosol, sometimes by as much as 3 logs.

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Selected References

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