Abstract
A microtechnique for determining antibiotic susceptibilities by the serial dilution method was evaluated in a clinical diagnostic microbiology laboratory. As compared with the standard tube method, an agreement of 94% was achieved for determining minimal inhibitory concentration with ± one tube dilution as the criterion of comparison. The experience with this system suggests that it could easily be incorporated into diagnostic laboratories as a routine procedure.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUER A. W., ROBERTS C. E., Jr, KIRBY W. M. Single disc versus multiple disc and plate dilution techniques for antibiotic sensitivity testing. Antibiot Annu. 1959;7:574–580. [PubMed] [Google Scholar]
- BEARGIE R. A., BRACKEN E. C., RILEY H. D., Jr MICROMETHOD (SPOT-PLATE) DETERMINATION OF IN VITRO ANTIBIOTIC SUSCEPTIBILITY. Appl Microbiol. 1965 Mar;13:279–280. doi: 10.1128/am.13.2.279-280.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRANCH A., FITZPATRICK K. M., GREENBERG L. The status of the antibiotic disc in Canada. Can Med Assoc J. 1957 Feb 1;76(3):194–198. [PMC free article] [PubMed] [Google Scholar]
- BRANCH A., STARKEY D. H., POWER E. E. The international situation with regard to the use of discs for antibiotic sensitivity tests. Antibiot Annu. 1958;6:833–835. [PubMed] [Google Scholar]
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Goss W. A., Cimijotti E. B. Evaluation of an automatic diluting device for microbiological applications. Appl Microbiol. 1968 Sep;16(9):1414–1416. doi: 10.1128/am.16.9.1414-1416.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harwick H. J., Weiss P., Fekety F. R., Jr Application of microtitration techniques to bacteriostatic and bactericidal antibiotic susceptibility testing. J Lab Clin Med. 1968 Sep;72(3):511–516. [PubMed] [Google Scholar]
- Petersdorf R. G., Sherris J. C. Methods and significance of in vitro testing of bacterial sensitivity to drugs. Am J Med. 1965 Nov;39(5):766–779. doi: 10.1016/0002-9343(65)90096-3. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]


