Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1969 Jun;17(6):914–919. doi: 10.1128/am.17.6.914-919.1969

T Antigens of Bovine and Canine Adenoviruses Demonstrated by Immunofluorescence

Jerome Kern a, Raymond V Gilden a, Robert J Huebner a,1
PMCID: PMC377839  PMID: 4240433

Abstract

The intracellular development in acutely infected cells of bovine and canine adenovirus T antigens was followed by immunofluorescent staining. With both species of adenovirus, antigen was first detected as intranuclear pin-points at 18 hr postinfection and coalescence into large lobular masses was noticed by 24 hr. Cross-reactions between bovine 1 (nononcogenic) and bovine 3 (oncogenic) T antigens were not observed by the direct technique although the more sensitive indirect procedure did reveal cross-reactivity. Extensive cross-reactions were observed between the T antigens of the oncogenic canine hepatitis virus and the ”nononcogenic” Toronto strain of canine adenovirus. The magnitude of these reactions places the two canine strains in the same T antigen subgroup. The canine and bovine T antigens were not stained by tumor antisera against any of the known human or simian T antigen subgroups. Antigen synthesis was not prevented by inhibitors of deoxyribonucleic acid synthesis although the appearance was altered markedly.

Full text

PDF
914

Images in this article

915Image
on p.915
916Image
on p.916
916Image
on p.916
918Image
on p.918

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
  2. Darbyshire J. H. Oncogenicity of bovine adenovirus type 3 in hamsters. Nature. 1966 Jul 2;211(5044):102–102. doi: 10.1038/211102a0. [DOI] [PubMed] [Google Scholar]
  3. Ditchfield J., Macpherson L. W., Zbitnew A. Association of Canine Adenovirus (Toronto A 26/61) with an Outbreak of Laryngotracheitis ("Kennel Cough"): A Preliminary Report. Can Vet J. 1962 Aug;3(8):238–247. [PMC free article] [PubMed] [Google Scholar]
  4. Feldman L. A., Rapp F. Inhibition of adenovirus replication by 1-beta-D-arabinofuranosylcytosine. Proc Soc Exp Biol Med. 1966 May;122(1):243–247. [PubMed] [Google Scholar]
  5. Gilden R. V., Beddow T. G., Huebner R. J. Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens. Appl Microbiol. 1967 May;15(3):657–660. doi: 10.1128/am.15.3.657-660.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilden R. V., Kern J., Beddow T. G., Huebner R. J. Bovine adenovirus type 3: detection of specific tumor and T antigens. Virology. 1967 Apr;31(4):727–729. doi: 10.1016/0042-6822(67)90205-x. [DOI] [PubMed] [Google Scholar]
  7. Gilden R. V., Kern J., Lee Y. K., Huebner R. J. Biological separation of adenovirus T and V antigen synthesis in KB cells. Proc Soc Exp Biol Med. 1968 Feb;127(2):639–642. doi: 10.3181/00379727-127-32762. [DOI] [PubMed] [Google Scholar]
  8. Gilead Z., Ginsberg H. S. Characterization of a Tumorlike Antigen in Type 12 and Type 18 Adenovirus-Infected Cells. J Bacteriol. 1965 Jul;90(1):120–125. doi: 10.1128/jb.90.1.120-125.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KLEIN M., EARLEY E., ZELLAT J. Isolation from cattle of a virus related to human adenovirus. Proc Soc Exp Biol Med. 1959 Oct;102:1–4. doi: 10.3181/00379727-102-25123. [DOI] [PubMed] [Google Scholar]
  10. Lewis A. M., Wiese W. H., Rowe W. P. The presence of antibodies in human serum to early (T) adenovirus antigens. Proc Natl Acad Sci U S A. 1967 Mar;57(3):622–629. doi: 10.1073/pnas.57.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
  13. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  15. Sarma P. S., Vass W., Huebner R. J., Igel H., Lane W. T., Turner H. C. Induction of tumours in hamsters with infectious canine hepatitis virus. Nature. 1967 Jul 15;215(5098):293–294. doi: 10.1038/215293a0. [DOI] [PubMed] [Google Scholar]
  16. Yamamoto T. Some physical and growth characteristics of a canine adenovirus isolated from dogs with laryngotracheitis. Can J Microbiol. 1966 Apr;12(2):303–311. doi: 10.1139/m66-041. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES