Figure 1. Cryo-electron tomograms of a reconstituted COPII budding reaction. Scale bars = 100 nm.
(A) A slice through a tomogram showing two coat layers arranged around tubular and spherical membranes. White, black and blue arrows point to the membrane, inner, and outer coat layers respectively. (B) A slice through the top of the tubes in panel A, showing repeating features in the coat layers. (C) A surface rendering of a COPII-coated tube. The membrane and inner coat are in grey, the outer coat in blue. (D) Surface renderings of spherically curved regions of membrane, coloured as in panel C.