Abstract
A Staphylococcus strain was inoculated on the top and cut surfaces of freshly baked Southern custard pies which were then packaged in a pasteboard carton and held at 30 C. Daily plate counts of surface sections 0.3 inch (0.76 cm) in thickness were made. The top surface inoculum showed a 24-hr lag time. This was due to the protective action of a top cakelike layer as shown by homogenization of the mix and coating of the surface. Substitution of all sweeteners with dextrose completely inhibited growth on the top surface. Further addition of dextrose to lower water activity (Aw) to 0.9 prevented growth on the cut surface as well, but such pies were organoleptically unacceptable. Growth on the top surface could also be prevented by 80 μg of undissociated sorbic acid per g in combination with 100 μg of undissociated propionic acid per g in the baked pie. Growth on the cakelike top surface was always retarded longer than on the cut surface provided the packaging allowed evaporation of surface moisture. Reducing the Aw of a different type of cream pie to 0.907 prevented top surface growth. It was concluded that baked cream pies with a cakelike top layer could be marketed with a “refrigerate after opening” label, provided the package maintains the moisture gradient caused by the surface skin and either a combination of 80 μg of undissociated sorbic acid per g and 100 μg undissociated propionic acid per g is present in the baked pie or the Aw of the baked pie is 0.920 or lower.
Full text
PDF







Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANGELOTTI R., FOTER M. J., LEWIS K. H. Time-temperature effects on Salmonellae and Staphylococci in foods. III. Thermal death time studies. Appl Microbiol. 1961 Jul;9:308–315. doi: 10.1128/am.9.4.308-315.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ANGELOTTI R., FOTER M. J., LEWIS K. H. Time-temperature effects on salmonellae and staphylococci in foods. I. Behavior in refrigerated foods. II. Behavior at warm holding temperatures. Am J Public Health Nations Health. 1961 Jan;51:76–88. doi: 10.2105/ajph.51.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRISLEY F. D., ANGELOTTI R., FOTER M. J. MULTIPLICATION OF STAPHYLOCOCCUS AUREUS IN SYNTHETIC CREAM FILLINGS AND PIES. Public Health Rep. 1964 May;79:369–376. [PMC free article] [PubMed] [Google Scholar]
- Dauer Carl C. 1960 summary of disease outbreaks and a 10-Year résumé. Public Health Rep. 1961 Oct;76(10):915–922. [PMC free article] [PubMed] [Google Scholar]
- Genigeorgis C., Sadler W. W. Effect of sodium chloride and pH on enterotoxin B production. J Bacteriol. 1966 Nov;92(5):1383–1387. doi: 10.1128/jb.92.5.1383-1387.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IANDOLO J. J., ORDAL Z. J., WITTER L. D. THE EFFECT OF INCUBATION TEMPERATURE AND CONTROLLED PH ON THE GROWTH OF STAPHYLOCOCCUS AUREUS MF 31 AT VARIOUS CONCENTRATIONS OF NACL. Can J Microbiol. 1964 Oct;10:808–811. doi: 10.1139/m64-104. [DOI] [PubMed] [Google Scholar]
- PETERSON A. C., BLACK J. J., GUNDERSON M. F. STAPHYLOCOCCI IN COMPETITION. III. INFLUENCE OF PH AND SALT ON STAPHYLOCOCCAL GROWTH IN MIXED POPULATIONS. Appl Microbiol. 1964 Jan;12:70–76. doi: 10.1128/am.12.1.70-76.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TROLLER J. A., FRAZIER W. C. Repression of Staphylococcus aureus by food bacteria. I. Effect of environmental factors on inhibition. Appl Microbiol. 1963 Jan;11:11–14. doi: 10.1128/am.11.1.11-14.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

