Abstract
A method for the detection of deoxyribonuclease activity in semisolid agar cultures was investigated. When cultures were overlaid with an acridine orangedeoxyribonucleate-agar (ADA) mixture, incubated for 1 to 3 hr, and observed under ultraviolet light, clear halos developed around colonies that produced deoxyribonuclease. A variation of the method for use with broth cultures involved impregnation of filter-paper discs with a small portion of the culture and overlaying the discs with the ADA mixture. This alteration has the advantage that the test tube cultures are easily heat-treated prior to assay to determine the heat resistance of the enzyme.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- JEFFRIES C. D., HOLTMAN D. F., GUSE D. G. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957 Apr;73(4):590–591. doi: 10.1128/jb.73.4.590-591.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lanyi J. K., Lederberg J. Fluorescent method for the detection of excreted ribonuclease around bacterial colonies. J Bacteriol. 1966 Nov;92(5):1469–1472. doi: 10.1128/jb.92.5.1469-1472.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WECKMAN B. G., CATLIN B. W. Deoxyribonuclease activity of micrococci from clinical sources. J Bacteriol. 1957 Jun;73(6):747–753. doi: 10.1128/jb.73.6.747-753.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]