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. 1969 Aug;18(2):262–267. doi: 10.1128/am.18.2.262-267.1969

Effect of Cultural Environment on the Blood Group Activity of Microorganisms1

Marcia R Moody 1,2, Viola M Young 1,2, John E Faber 1,2
PMCID: PMC377954  PMID: 4896884

Abstract

Blood group activity was proven to be a property of the bacterium per se which possesses it, although such activity was influenced by the cultural environment of the organism. High concentrations of peptones having blood group activity were able to transfer this activity to inactive organisms; however, the conferred activity was proportional to the concentration. As a result of the low concentrations, the blood group activity of peptones was eliminated upon incorporation in culture media, and the activity of the peptones had no effect on the blood group activity levels of microorganisms when grown in these media. Conversely, the vitamin content of culture media did affect blood group active organisms. Multiple vitamins in media decreased the activity levels by blocking the reactive sites of the active organisms on which activity detection depended. Blood group activity levels were highest in media of minimal or no vitamin content. Therefore, it can be concluded that a choice of cultural medium becomes an important factor in the quantitation of bacterial blood group activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eyquem A., Le Minor L. Recherches sur les communautés antigéniques entre les substances de groupes sanguins et les entérobactéries. Ann Inst Pasteur (Paris) 1965 Nov;(Suppl):85–93. [PubMed] [Google Scholar]
  2. FENTON J. W., 2nd, DUGGLEBY C. R., OTTEN C., STONE W. H. ISOLATION AND FLUORESCENT LABELING OF ULEX EUROPAEUS ANTI-H LECTIN. Vox Sang. 1965 Mar-Apr;10:208–211. doi: 10.1111/j.1423-0410.1965.tb04339.x. [DOI] [PubMed] [Google Scholar]
  3. GONANO F., MODIANO G., DE ANDREIS M. Relationships between the somatic antigens of E. coli O86 B:7 and blood group A and B substances. Vox Sang. 1961 Nov;6:685–691. doi: 10.1111/j.1423-0410.1961.tb04055.x. [DOI] [PubMed] [Google Scholar]
  4. LANDAU J. W., FUJIWARA A., NEWCOMER V. D., DZAWACHISZWILI N. THE RELATIONSHIP OF COMMONLY ENCOUNTERED SAPROPHYTIC AND PATHOGENIC FUNGI TO HUMAN A AND B BLOOD GROUP ANTIGENS. J Invest Dermatol. 1964 Jul;42:59–70. [PubMed] [Google Scholar]
  5. LAYRISSE M., PERES Z., AGUERO O., PITTALUGA J. R. In vitro and in vivo effect of ascorbic acid, cortisone and several chemical substances containing-SH radicals, upon the agglutinins, especially anti-Rh agglutinins. Rev Belg Pathol Med Exp. 1955 Mar;24(2):122–127. [PubMed] [Google Scholar]
  6. Lee M. R., Ikari N. S., Branche W. C., Jr, Young V. M. Microtiter bacterial hemagglutination technique for detection of Shigella antibodies. J Bacteriol. 1966 Jan;91(1):463–463. doi: 10.1128/jb.91.1.463-.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MUSCHEL L. H., OSAWA E. Human blood group substance B and Escherichia coli 086. Proc Soc Exp Biol Med. 1959 Aug-Sep;101:614–617. doi: 10.3181/00379727-101-25036. [DOI] [PubMed] [Google Scholar]
  8. ROWLEY D., JENKIN C. R. Antigenic cross-reaction between host and parasite as a possible cause of pathogenicity. Nature. 1962 Jan 13;193:151–154. doi: 10.1038/193151a0. [DOI] [PubMed] [Google Scholar]
  9. SPRINGER G. F. Inhibition of blood-group agglutinins by substances occurring in plants. J Immunol. 1956 Jun;76(6):399–407. [PubMed] [Google Scholar]
  10. SPRINGER G. F., ROSE C. S., GYORGY P. Blood group mucoids: their distribution and growth-promoting properties for Lactobacillus bifidus var. Penn. J Lab Clin Med. 1954 Apr;43(4):532–542. [PubMed] [Google Scholar]
  11. SPRINGER G. F., WILLIAMSON P., READLER B. L. Blood group active gram-negative bacteria and higher plants. Ann N Y Acad Sci. 1962 May 3;97:104–110. doi: 10.1111/j.1749-6632.1962.tb34626.x. [DOI] [PubMed] [Google Scholar]
  12. WRIGHT G. G., FEINBERG R. J. Hemagglutination by tularemia antisera: further observations on agglutination of polysaccharide-treated erythrocytes and its inhibition by polysaccharide. J Immunol. 1952 Jan;68(1):65–71. [PubMed] [Google Scholar]

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