Fig. 5.

Comparison of TAG detection in C. kessleri UTEX 2229, C. sorokiniana CS-01, C. sorokiniana UTEX 1230 under BBM and BBM-N (a). Separation of neutral lipid from polar lipid and glycolipid, and authentic standards by HPLC–ELSD. 1 triacylglycerols (TAG, 1 μg), 2 diacylglycerol (DAG) ester 3 diacylglycerol (DAG, 4 μg), 4 free fatty acids (palmitic acid 2 μg), 5 phosphaphatidylcholine (PtdCho, 20 μg), 6 monogalactosyldiacylglycerol (MGDG, 20 μg), 7 digalactosyldiacylglycerol (DGDG, 40 μg), 8 carotenoids extracted from C. reinhardtii. (b) TAG contents of C. kessleri UTEX 2229, C. sorokiniana CS-01 and UTEX 1230 under BBM and BBM-N by HPLC–ELSD. Error bar n = 3 technical replicates from one 3-L bioreactor culture. Ave. of % DW ± standard deviation