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. 1969 Sep;18(3):355–359. doi: 10.1128/am.18.3.355-359.1969

Evaluation of Media for Differentiating Nonfermenting Gram-negative Bacteria of Medical Significance

Gerald L Gilardi 1
PMCID: PMC377984  PMID: 4907000

Abstract

An evaluation was made of media and tests used for differentiating nonfermenting gram-negative bacteria encountered in medical bacteriology in order to determine those diagnostic procedures most useful in identifying these bacteria. The organisms examined included Alcaligenes faecalis, A. odorans var. viridans, Moraxella duplex (Mima polymorpha var. oxidans), Acinetobacter anitratum (Herellea vaginicola), A. lwoffi (Mima polymorpha), Pseudomonas fluorescens, P. putida, P. maltophilia, P. pseudomallei, P. stutzeri, P. alcaligenes, and atypical strains of P. aeruginosa. The media and tests evaluated included Sellers' medium; Hugh and Leifson's OF medium; acid production from 10% lactose infusion agar; gluconate oxidation; starch, aesculin, and Tween 80 hydrolysis; lysine decarboxylase, arginine dihydrolase, deoxyribonuclease, and tyrosinase activity; tolerance to triphenyl tetrazolium chloride, cetrimide, cadmium sulfate, 2.5% and 6.5% sodium chloride, and pH 5.6; utilization of glucose, acetamide, and malonate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann P., Doudoroff M., Stanier R. Y. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968 May;95(5):1520–1541. doi: 10.1128/jb.95.5.1520-1541.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown V. I., Lowbury E. J. Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa. J Clin Pathol. 1965 Nov;18(6):752–756. doi: 10.1136/jcp.18.6.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elston H. R., Elston J. H. Further use of deoxyribonuclease in a screening test for Serratia. J Clin Pathol. 1968 Mar;21(2):210–212. doi: 10.1136/jcp.21.2.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farkas-Himsley H. Evaluation of Sellers medium in identification of some gram-negative bacteria. Appl Microbiol. 1967 May;15(3):661–664. doi: 10.1128/am.15.3.661-664.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GABY W. L., FREE E. Differential diagnosis of Pseudomonas-like microorganisms in the clinical laboratory. J Bacteriol. 1958 Oct;76(4):442–444. doi: 10.1128/jb.76.4.442-444.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilardi G. L. Diagnostic criteria for differentiation of pseudomonads pathogenic for man. Appl Microbiol. 1968 Oct;16(10):1497–1502. doi: 10.1128/am.16.10.1497-1502.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilardi G. L. Differentiation of oxidative and nonglucolytic Gram-negative bacteria. Am J Med Technol. 1968 Jun;34(6):334–345. [PubMed] [Google Scholar]
  8. Gilardi G. L. Morphological and biochemical differentiation of Achromobacter and Moraxella (DeBord's tribe Mimeae). Appl Microbiol. 1968 Jan;16(1):33–38. doi: 10.1128/am.16.1.33-38.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godes J. R. An evaluation of media for differentiating nonfermenting gram negative bacteria. Am J Med Technol. 1967 Jul-Aug;33(4):311–322. [PubMed] [Google Scholar]
  10. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hedberg M. Acetamide agar medium selective for Pseudomonas aeruginosa. Appl Microbiol. 1969 Mar;17(3):481–481. doi: 10.1128/am.17.3.481-481.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEIFSON E., HUGH R. Alcaligenes denitrificans n.sp. J Gen Microbiol. 1954 Dec;11(3):512–513. doi: 10.1099/00221287-11-3-512. [DOI] [PubMed] [Google Scholar]
  13. LYSENKO O. Pseudomonas--an attempt at a general classification. J Gen Microbiol. 1961 Jul;25:379–408. doi: 10.1099/00221287-25-3-379. [DOI] [PubMed] [Google Scholar]
  14. Leifson E. The Fermentation of Sodium Malonate as a Means of Differentiating Aerobacter and Escherichia. J Bacteriol. 1933 Sep;26(3):329–330. doi: 10.1128/jb.26.3.329-330.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
  16. Pease M., Malcolm J., Chernaik R., Dunlop S. An approach to the problem of differentiating pseudomonads in the clinical laboratory. Am J Med Technol. 1968 Jan;34(1):35–40. [PubMed] [Google Scholar]
  17. Redfearn M. S., Palleroni N. J., Stanier R. Y. A comparative study of Pseudomonas pseudomallei and Bacillus mallei. J Gen Microbiol. 1966 May;43(2):293–313. doi: 10.1099/00221287-43-2-293. [DOI] [PubMed] [Google Scholar]
  18. SELLERS W. MEDIUM FOR DIFFERENTIATING THE GRAM-NEGATIVE, NONFERMENTING BACILLI OF MEDICAL INTEREST. J Bacteriol. 1964 Jan;87:46–48. doi: 10.1128/jb.87.1.46-48.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SIERRA G. A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie Van Leeuwenhoek. 1957;23(1):15–22. doi: 10.1007/BF02545855. [DOI] [PubMed] [Google Scholar]
  20. Snodgrass C. J., Koburger J. A. Response of the Mimeae to some physical and chemical agents. Appl Microbiol. 1968 Nov;16(11):1776–1778. doi: 10.1128/am.16.11.1776-1778.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]

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