Abstract
Except for Mycoplasma fermentans strain PG 18, single-cell suspensions of M. arthritidis, M. fermentans (ATCC 19989), M. hominis type 1, M. orale types 1 and 2, M. pneumoniae, and M. salivarium were inactivated exponentially by ultraviolet (UV) irradiation, in contrast to broth cultures containing clusters of elementary bodies. The susceptibility of the mycoplasmas was unaffected by storage at 2-4 C and at -70 C, by sonication, and by filtration. The rate of inactivation was dependent on the intensity of the radiations but independent of the concentration of the cells. Therefore, single-cell suspensions of these mycoplasmas could be differentiated from aggregates of cells by exponential inactivation of the colony-forming units (CFU). By this criterion, the CFU of M. arthritidis in the exponential phase of growth consisted of single cells, in contrast to the other species in which the CFU contained two or more elementary bodies. Even though the cultures of M. fermentans (PG 18) were grown from single cells, they were not homogeneous in their susceptibility to UV light. Neither were cultures of M. arthritidis and M. orale type 1 grown from single cells which had survived irradiation.
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Selected References
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- BARILE M. F., YAGUCHI R., EVELAND W. C. A simplified medium for the cultivation of pleuropneumonia-like organisms and the L-forms of bacteria. Am J Clin Pathol. 1958 Aug;30(2):171–176. doi: 10.1093/ajcp/30.2_ts.171. [DOI] [PubMed] [Google Scholar]
- CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
- Clark H. W. Sedimentation counting and morphology of Mycoplasma. J Bacteriol. 1965 Nov;90(5):1373–1386. doi: 10.1128/jb.90.5.1373-1386.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Furness G. Analysis of the growth cycle of Mycoplasma orale by synchronized division and by ultraviolet irradiation. J Infect Dis. 1968 Oct;118(4):436–442. doi: 10.1093/infdis/118.4.436. [DOI] [PubMed] [Google Scholar]
- Furness G., Pipes F. J., McMurtrey M. J. Analysis of the life cycle of Mycoplasma pneumoniae by synchronized division and by ultraviolet and x irradiations. J Infect Dis. 1968 Feb;118(1):7–13. doi: 10.1093/infdis/118.1.7. [DOI] [PubMed] [Google Scholar]
- Furness G., Pipes F. J., McMurtrey M. J. Susceptibility of human mycoplasmata to ultraviolet and x irradiations. J Infect Dis. 1968 Feb;118(1):1–6. doi: 10.1093/infdis/118.1.1. [DOI] [PubMed] [Google Scholar]
- Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
- Kundsin R. B. Characterization of Mycoplasma aerosols as to viability, particle size, and lethality of ultraviolet irradiation. J Bacteriol. 1966 Mar;91(3):942–944. doi: 10.1128/jb.91.3.942-944.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanbridge E., Hayflick L. Growth inhibition test for identification of Mycoplasma species utilizing dried antiserum-impregnated paper discs. J Bacteriol. 1967 Apr;93(4):1392–1396. doi: 10.1128/jb.93.4.1392-1396.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warren J. Observations on Some Biological Characteristics of Organisms of the Pleuropneumonia Group. J Bacteriol. 1942 Feb;43(2):211–228. doi: 10.1128/jb.43.2.211-228.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]