Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1969 Oct;18(4):535–541. doi: 10.1128/am.18.4.535-541.1969

Selection of Media for the Isolation of Common Bacterial L-Phase Organisms from a Clinical Specimen

Lynn N Nimmo 1, Donna J Blazevic 1
PMCID: PMC378030  PMID: 4905032

Abstract

The L-phase of 13 bacteria commonly associated with disease were induced by penicillin and inoculated into various solid and broth media; their growth was recorded for a period of 14 days. Plates containing highly purified agar and sucrose as the stabilizing agent and those incubated under aerobic conditions gave the best results. Magnesium seems to be necessary for growth in broth media on primary isolation, although it may not be necessary on multiple transfers after a more stable state has been reached. Growth in broth media is much more difficult to achieve. Reversion is aided by using a higher concentration of agar in plates, by decreasing the sucrose concentration, and by omitting the antibiotics and horse serum. A procedure has been outlined for the routine culture and identification of L-phase organisms from a clinical specimen.

Full text

PDF
535

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRAWFORD Y. E., FRANK P. F., SULLIVAN B. Isolation and reversion of L forms of beta-hemolytic streptococci. J Infect Dis. 1958 Jan-Feb;102(1):44–52. doi: 10.1093/infdis/102.1.44. [DOI] [PubMed] [Google Scholar]
  2. Davis S. D., Gemsa D., Wedgwood R. J. Kinetics of the transformation of Gram-negative rods to spheroplasts and ghosts by serum. J Immunol. 1966 Apr;96(4):570–577. [PubMed] [Google Scholar]
  3. Dienes L., Bullivant S. Comparison of the morphology of PPLO and L-forms of bacteria with light and electron microscopy. Ann N Y Acad Sci. 1967 Jul 28;143(1):719–733. doi: 10.1111/j.1749-6632.1967.tb27719.x. [DOI] [PubMed] [Google Scholar]
  4. GODZESKI C. W., BRIER G., GRIFFITH R. S., BLACK H. R. ASSOCIATION OF BACTERIAL L-PHASE ORGANISMS IN CHRONIC INFECTIONS. Nature. 1965 Mar 27;205:1340–1340. doi: 10.1038/2051340a0. [DOI] [PubMed] [Google Scholar]
  5. GODZESKI C. W., BRIER G., PAVEY D. E. Bacterial L-phase growth as a possible complicating factor in certain bacteriological procedures. Life Sci. 1962 Nov;1:565–569. doi: 10.1016/0024-3205(62)90086-3. [DOI] [PubMed] [Google Scholar]
  6. GRAYKOWSKI E. A., BARILE M. F., STANLEY H. R. PERIADENITIS APHTHAE: CLINICAL AND HISTOPATHOLOGIC ASPECTS OF LESIONS IN A PATIENT AND OF LESIONS PRODUCED IN RABBIT SKIN. J Am Dent Assoc. 1964 Aug;69:118–126. doi: 10.14219/jada.archive.1964.0242. [DOI] [PubMed] [Google Scholar]
  7. GUZE L. B., KALMANSON G. M. PERSISTENCE OF BACTERIA IN "PROTOPLAST" FORM AFTER APPARENT CURE OF PYELONEPHRITIS IN RATS. Science. 1964 Mar 20;143(3612):1340–1341. [PubMed] [Google Scholar]
  8. Godzeski C., Brier G., Glenn W. L-phase bacteria in human heart tissue. Life Sci. 1968 Jan 15;7(2):107–112. doi: 10.1016/0024-3205(68)90353-6. [DOI] [PubMed] [Google Scholar]
  9. Gutman L. T., Turck M., Petersdorf R. G., Wedgwood R. J. Significance of bacterial variants in urine of patients with chronic bacteriuria. J Clin Invest. 1965 Dec;44(12):1945–1952. doi: 10.1172/JCI105300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamburger M., Carleton J. Staphylococcal spheroplasts and L colonies. I. Population curves of vegetative staphylococci and spheroplasts in methicillin-containing broth over long periods. J Infect Dis. 1966 Apr;116(2):221–230. doi: 10.1093/infdis/116.2.221. [DOI] [PubMed] [Google Scholar]
  11. Hamburger M., Carleton J. Staphylococcal spheroplasts and L colonies. II. Conditions conducive to reversion of spheroplasts to vegetative staphylococci. J Infect Dis. 1966 Dec;116(5):544–550. doi: 10.1093/infdis/116.5.544. [DOI] [PubMed] [Google Scholar]
  12. KAGAN C. Y., MIKHAILOVA V. S. ISOLATION OF L-FORMS OF STREPTOCOCCI FROM THE BLOOD OF PATIENTS WITH RHEUMATISM AND ENDOCARDITIS. J Hyg Epidemiol Microbiol Immunol. 1963;7:327–343. [PubMed] [Google Scholar]
  13. Kang K. S., Casida L. E., Jr Large bodies of Mycoplasma and L-form organisms. J Bacteriol. 1967 Mar;93(3):1137–1142. doi: 10.1128/jb.93.3.1137-1142.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MATTMAN L. H., MATTMAN P. E. L FORMS OF STREPTOCOCCUS FECALIS IN SEPTICEMIA. Arch Intern Med. 1965 Mar;115:315–321. doi: 10.1001/archinte.1965.03860150059011. [DOI] [PubMed] [Google Scholar]
  15. MATTMAN L. H., TUNSTALL L. H., ROSSMOORE H. W. Induction and characteristics of staphylococcal L forms. Can J Microbiol. 1961 Oct;7:705–713. doi: 10.1139/m61-084. [DOI] [PubMed] [Google Scholar]
  16. Roberts R. B. L form of Neisseria gonorrhoeae. J Bacteriol. 1966 Dec;92(6):1609–1614. doi: 10.1128/jb.92.6.1609-1614.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES