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. 1969 Oct;18(4):604–611. doi: 10.1128/am.18.4.604-611.1969

Radiation Sterilization of Prototype Military Foods. III. Pork Loin

Abe Anellis 1, D Berkowitz 1, C Jarboe 1, H M El-Bisi 1
PMCID: PMC378045  PMID: 4905038

Abstract

Ten lots of pork loin, packed in cans, were inoculated with approximately 106Clostridium botulinum spores per can. Each lot was seeded with a different strain; five type A and five type B strains were used. The pack comprised 5,690 cans, including controls, and contained about 109 spores per dose. The cans were irradiated with Co60 in the range of 0 to 5.0 Mrad (0.5 Mrad increments) at 5 to 25 C, incubated for 6 months at 30 C, and examined for swelling, toxicity, and recoverable C. botulinum. The minimal experimental sterilizing dose (ESD) based on nonswollen, nontoxic, but nonsterile end points was 2.5 < ESD ≤ 3.0 Mrad, and based on non-spoiled sterile cans was 3.5 < ESD ≤ 4.0 Mrad. The theoretical minimal radiation dose (MRD), the 12D equivalent, varied with the method of computation: 4.74, 4.33 ± 0.17, and 4.19 to 4.99 Mrad were obtained by the Weibull, Spearman-K̈arber, and Schmidt-Nank techniques, respectively. Calculation of D and MRD values by the conventional Schmidt-Nank method produced increasing values with rising dosage; this finding was compared with the data derived by the other two methods of calculation. Suggestions for estimating the MRD of a prototype radiation process are offered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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