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. 1969 Oct;18(4):612–617. doi: 10.1128/am.18.4.612-617.1969

Immunofluorescent Staining of Salmonella Species with Flagellar Sera1

J M Goepfert 1,2, Ruthann Hicks 1,2
PMCID: PMC378046  PMID: 4189824

Abstract

Salmonellae stained with flagellar sera by either the direct or indirect fluorescent-antibody technique fluoresced at the cell surface. Investigations showed that fimbriae, flagellar material, and capsule antigens were not the participating antigens in this reaction. Cell surface staining was inhibited by mannose but was unaffected by glucose, galactose, lactose, maltose, and sucrose. Specific absorption of the flagellar sera by intact cells and purified somatic antigen showed that O antigens were the site of the surface staining. The advantages of using flagellar sera in fluorescent-antibody screening of materials for the presence of salmonellae are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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