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. 1969 Nov;18(5):810–815. doi: 10.1128/am.18.5.810-815.1969

Production of Interferon in Serum-Free Human Leukocyte Suspensions1

H Strander a,2
PMCID: PMC378093  PMID: 4313169

Abstract

The recovery of interferon from Sendai-infected suspensions of purified human leukocytes is dependent on the serum concentration in the incubation medium. Very little interferon is obtained from serum-free suspensions. The data reported demonstrate that the critical macromolecular, age-independent, and species-unspecific serum principle can be omitted from the suspensions if the medium is supplemented with a combination of crystalline serum albumin and high concentrations of any one of five studied dipolar ionic buffers [N,N-bis(2-hydroxyethyl) glycine (Bicine), N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 2-(N-morpholino)ethanesulfonic acid (MES), N-tris(hydroxymethyl)methyl-2-amino-ethanesulfonic acid (TES), and N-tris(hydroxymethyl)methylglycine (Tricine)]. The optimal combination [TES (1.0%, w/v) and bovine serum albumin (0.8%, w/v)] allows the production of potent preparations of serum-free human interferon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantell K., Strander H., Saxén L., Meyer B. Interferon response of human leukocytes during intrauterine and postnatal life. J Immunol. 1968 Jun;100(6):1304–1309. [PubMed] [Google Scholar]
  2. Falcoff E., Lévy H., Colin J., Chany C. Estimation du poids moléculaire de l'interféron produit par les globules blancs humains. C R Acad Sci Hebd Seances Acad Sci D. 1965 May 17;260(20):5405–5407. [PubMed] [Google Scholar]
  3. Green I., Paul W. E., Benacerraf B. Hapten carrier relationships in the DNP-PLL foreign albumin complex system: induction of tolerance and stimulation of cells in vitro. J Exp Med. 1968 Jan 1;127(1):43–53. doi: 10.1084/jem.127.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Krueger R. G. The effect of Streptomycin on antibody synthesis in vitro. Proc Natl Acad Sci U S A. 1965 Jul;54(1):144–152. doi: 10.1073/pnas.54.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LAMPSON G. P., TYTELL A. A., NEMES M. M., HILLEMAN M. R. Purification and characterization of chick embryo interferon. Proc Soc Exp Biol Med. 1963 Feb;112:468–478. doi: 10.3181/00379727-112-28080. [DOI] [PubMed] [Google Scholar]
  6. Merigan T. C., Gregory D. F., Petralli J. K. Physical properties of human interferon prepared in vitro and in vivo. Virology. 1966 Aug;29(4):515–522. doi: 10.1016/0042-6822(66)90276-5. [DOI] [PubMed] [Google Scholar]
  7. PARROTT R. H., VARGOSKO A. J., KIMHW, BELL J. A., CHANOCK R. M. Acute respiratory diseases of viral etiology. III. parainfluenza. Myxoviruses. Am J Public Health Nations Health. 1962 Jun;52:907–917. doi: 10.2105/ajph.52.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Strander H., Cantell K. Further studies on the production of interferon by human leukocyte in vitro. Ann Med Exp Biol Fenn. 1967;45(1):20–29. [PubMed] [Google Scholar]
  9. Strander H., Cantell K. Production of interferon by human leukocytes in vitro. Ann Med Exp Biol Fenn. 1966;44(2):265–273. [PubMed] [Google Scholar]

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