Abstract
Ability to detect pigmented and nonpigmented Pasteurella pestis is essential in plague research, and is currently dependent on use of the synthetic hemin-agar of Jackson and Burrows. We have devised a new differential medium for this purpose, containing Congo red dye and common, commercially available laboratory media. The ease and simplicity of preparation make the Congo red-agar a practical routine laboratory tool in plague research. These findings, possibly indicating a common binding site for hematin and Congo red, should be useful in efforts to determine the chemical nature of a bacterial component associated with high virulence in P. pestis.
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- BURROWS T. W., BACON G. A. The effects of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis. Br J Exp Pathol. 1958 Jun;39(3):278–291. [PMC free article] [PubMed] [Google Scholar]
- BURROWS T. W. Genetics of virulence in bacteria. Br Med Bull. 1962 Jan;18:69–73. doi: 10.1093/oxfordjournals.bmb.a069938. [DOI] [PubMed] [Google Scholar]
- BURROWS T. W., JACKSON S. The pigmentation of Pasteurella pestis on a defined medium containing haemin. Br J Exp Pathol. 1956 Dec;37(6):570–576. [PMC free article] [PubMed] [Google Scholar]
- BURROWS T. W., JACKSON S. The virulence-enhancing effect of iron on nonpigmented mutants of virulent strains of Pasteurella pestis. Br J Exp Pathol. 1956 Dec;37(6):577–583. [PMC free article] [PubMed] [Google Scholar]
- BURROWS T. W. VIRULENCE OF PASTEURELLA PESTIS AND IMMUNITY TO PLAGUE. Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1963;37:59–113. doi: 10.1007/978-3-662-36742-1_2. [DOI] [PubMed] [Google Scholar]
- GIRARD G. L'IMMUNIT'E DANS L'INFECTION PESTEUSE. ACQUISITIONS APPORT'EES PAR 30 ANN'EES DE TRAVAUX SUR LA SOUCHE DE "PASTEURELLA PESTIS EV" (GIRARD ET ROBIC) Biol Med (Paris) 1963 Nov-Dec;52:631–731. [PubMed] [Google Scholar]
- Johnson J. L., Chilton W. S. Galactosamine glycan of Chondrococcus columnaris. Science. 1966 May 27;152(3726):1247–1248. doi: 10.1126/science.152.3726.1247. [DOI] [PubMed] [Google Scholar]
- KRAKOW J. S. AZOTOBACTER VINELANDII RIBONUCLEIC ACID POLYMERASE. I. INHIBITION BY CONGO RED. Biochim Biophys Acta. 1965 Apr 19;95:532–537. doi: 10.1016/0005-2787(65)90506-x. [DOI] [PubMed] [Google Scholar]
- LITTLE H. N., NEILANDS J. B. Binding of haematin by human serum albumin. Nature. 1960 Dec 10;188:913–915. doi: 10.1038/188913a0. [DOI] [PubMed] [Google Scholar]