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. 1969 Nov;18(5):897–900. doi: 10.1128/am.18.5.897-900.1969

Accelerated Plaque Formation by Fowlpox Virus in the Presence of Chymotrypsin

Bonnie B Asch a,1, George E Gifford a
PMCID: PMC378109  PMID: 4905607

Abstract

Chymotrypsin enhanced fowlpox virus plaque formation in chick embryo cell cultures. A simplified plaque assay for fowlpox virus is described. Plaques were produced in 3 days when chymotrypsin was included in a serum-free fluid overlay. Plaques were also produced in 5 to 6 days under an agar overlay when a medium containing fetal calf serum was employed. Kinetics of plaque formation were also studied, and it was shown that fowlpox virus plaque diameters grow at a linear rate.

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Selected References

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