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. 2013 Oct;195(2):331–348. doi: 10.1534/genetics.113.155382

Figure 1.

Figure 1

Precise, targeted DNA insertions mediated by homology-directed repair of TALEN-induced DSBs. In a single experiment, animals were injected simultaneously with RNA encoding a ben-1 TALEN pair and three different ssOligos encoding different restriction sites (NcoI, HindIII, and EcoRI) designed to replace the endogenous PsiI site (TTATAA) shown in red. Shown are examples of the HDR-mediated mutations resulting from the single set of injections. Mutations include all three precisely inserted restriction sites and two NHEJ-mediated mutations causing a deletion or an indel. The TALE recognition sequences within ben-1 are shown adjacent to the color-coded repeat variable diresidues (RVDs), which recognize specific nucleotides (nt) of the DNA target site according to the code shown in the key. The locations in ben-1 DNA of the 20-nt homology arms used in each ssOligo are designated by a green line. Each ssOligo is composed of only the 6-nt restriction site to be inserted and the flanking 20-nt homology arms.