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(a) Immunocytochemistry analysis. Cells were fixed and stained after 24 hours of treatment with GSK461364A at IC75 concentration. Cells were stained with DAPI, α-tubulin, and pHISH3. (b) Quantification. Top panel: pHISH3-positive cells. Bottom panel: % of microtubules disorganization. (c) pHISH3 detection by Western blot. Cells were harvested at different time points after treatment with GSK461364A at IC75 concentration. DAPI, 4′,6-diamino-2-phenylindole.
