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. 2013 Jun 1;34(10):2415–2423. doi: 10.1093/carcin/bgt191

Fig. 1.

Fig. 1.

Suppressive effects of DAPM on cell proliferation and Notch signaling in colon cancer cell lines. Human colon cancer cell lines HCT116 (Wt and p21 − /−) and SW480 were treated with the indicated concentration of DAPM, for either 48 or 72 h. (A) HCT116 and SW480 cell lines were treated with increasing concentrations of DAPM for 72 h. Cell viability was assessed using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. Each data point represent the mean value of triplicate samples. *P < 0.05 compared with dimethyl sulfoxide treatment (Student’s t-test). (B) Western blot analysis for the indicated proteins after 48 h of treatment of DAPM. The blots were reprobed using β-actin as a loading control. (C) HCT116 parental and p21 − /− cell lines were treated with increasing concentrations of DAPM for 48 h. The effects of DAPM on the Notch signaling pathway were evaluated by western blot analysis for the indicated proteins after 48 h of treatment with DAPM. The blots were reprobed using β-actin as a loading control. (D) Both cell lines were treated with increasing concentration of DAPM for 72 h. Cell viability was assessed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. Columns, mean of triplicate samples; bars, standard deviation. *P < 0.05 compared with HCT116 parental cells (Student’s t-test).