Figure 2. Inter-Golgi transport of Golgi resident glycosyl-transferase proteins.

HeLa cells expressing either GT-GFP and VSV-G, or ST-RFP were mixed and fused by acidic exposure (1 min, pH 5) and then monitored by confocal video-microscopy at 20°C. Cells were treated with CHX (100 μg/ml) 2 hr prior to fusion and during the imaging. Graphs show fluorescence intensity of markers within Golgi 2, 4, and 5 over time. Results are representative of three independent experiments.

DOI: http://dx.doi.org/10.7554/eLife.01296.004