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. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: Biochemistry. 2013 Sep 11;52(38):6702–6711. doi: 10.1021/bi401041v

Figure 7. Kinetics of processing of 3'-OH versus 3'-PO4 substrates.

Figure 7

Phosphorolysis of 3' 32P-labeled 25-mer RNAp or RNAOH substrates by full-length PNPase in the presence of magnesium (panel A) or manganese (panel B) was assayed as described in Experimental Procedures. The reactions were quenched at the times specified and the products were analyzed by PEI-cellulose TLC. The extent of 32P-CDP release (as % of total radioactivity) is plotted as a function of reaction time. Each datum in the graphs is the average of three separate experiments ±SEM.