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Amino acid sequences of αB-crystallin and biotinyl αA66-80 (SDRDKFVIFLDVKHFK-Biotin). Sequences in blue denote C-terminal extension (151–175) region in αB-crystallin. Sequences in red denote the chaperone site in αB-crystallin. The underlined sequences in αB-crystallin are presumptive peptide interaction region identified by the GPMAW from the LC-MS data. Sequences underlined in green are confirmed by MS/MS.
