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. 1997 Oct;50(5):266–268. doi: 10.1136/mp.50.5.266

A rapid RT-PCR based method for the detection of BCR-ABL translocation.

Sidorova JYu 1, L B Saltykova 1, A A Lyschov 1, Zaritskey AYu 1, K M Abdulkadyrov 1, M N Blinov 1
PMCID: PMC379644  PMID: 9497918

Abstract

AIMS: To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection. METHODS: Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simultaneously in the same tube. RESULTS: On testing 40 chronic myelogenous leukaemia patients and 10 healthy donors there was a specificity for the newly developed technique. In addition, dilution experiments demonstrated that the protocol was highly sensitive. CONCLUSIONS: The suggested one step PCR strategy is a simple and reliable way to reveal BCR-ABL chimaeras.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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