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Dissociation constants for the interaction of 19F-Man3 with single binding site CV-N variants determined by ligand-observed 19F-NMR, reverse ITC, protein-observed NMR, and direct ITC experiments.
| Protein | KDF (μM) 19F-NMR | KDF (μM) reverse ITC | KDF (μM) 1H-15N HSQC | KDF (μM) direct ITC |
|---|---|---|---|---|
| [CVNΔA]ssm | 10.8 ± 0.3 | 12.6 ± 1.2 | 63.5 ± 5 | 62.5 ± 2 |
| CVNmDB | 33.5± 3.5 | 28.5 ± 3.5 | 150.3 ± 7 | 153.3 ± 5 |
