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. 1973 Jul;26(1):14–17. doi: 10.1128/am.26.1.14-17.1973

Gamma Radiation Inactivation of Coxsackievirus B-2

Robert Sullivan 1,2, Pasquale V Scarpino 1,2, Alexander C Fassolitis 1,2, Edward P Larkin 1,2, James T Peeler 1,2
PMCID: PMC379708  PMID: 4737851

Abstract

The radioresistance of coxsackievirus B-2 was studied when the virus was suspended in Eagle minimal essential medium, distilled water, cooked ground beef, and raw ground beef and irradiated at various temperatures in a cobalt-60 gamma radiation source. The number of surviving viruses at given doses of radiation was determined by a plaque assay system. All destruction curves indicated a first-order reaction. When the virus was irradiated in minimal essential medium at temperatures of -30, -60, and -90 C, D values (in Mrad) were 0.69, 0.59, and 0.64, respectively. When the virus was suspended in water and irradiated at -90 C, the D value was 0.53. Cooked ground beef containing the virus was irradiated at temperatures ranging from 16 to -90 C. The D values were 0.70 (16 C), 0.76 (0.5 C), 0.68 (-30 C), 0.78 (-60 C), and 0.81 (-90 C). Raw ground beef containing the virus was irradiated at -30, -60, and -90 C, and the D values were respectively 0.75, 0.71, and 0.68. The D values indicate that the rate of viral inactivation was dependent on the suspending menstrum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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