Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1973 Aug;26(2):196–201. doi: 10.1128/am.26.2.196-201.1973

Evaluation of New Urinary Tract Infection Screening Devices

William A Craig 1,2, Calvin M Kunin 1,2, Jane DeGroot 1,2
PMCID: PMC379750  PMID: 4582815

Abstract

Several new methods for detection of bacteriuria were studied to evaluate their usefulness as screening procedures. A new filter paper device incorporating dehydrated media and tetrazolium was found to be reliable when compared with the standard pour plate method in the laboratory and with the dip-slide method in a field test. It failed to detect yeasts and slowly growing streptococci. Antibiotics blocked the test when susceptible organisms were present. An agar-cup method was found to be quite reliable, but could be improved by use of differential media. The Griess test was confirmed in a small trial to be highly specific when used in conjunction with a first morning specimen, but of little value with random specimens. Phenzopyridine was found to give false positive reactions. The subnormal glucose test, although highly sensitive and specific, gave too many false positive tests to be useful other than as a screening method.

Full text

PDF
196

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen S. N., Kass E. H. A simple method for quantitative urine culture. N Engl J Med. 1967 Jul 27;277(4):176–180. doi: 10.1056/NEJM196707272770403. [DOI] [PubMed] [Google Scholar]
  2. Craig W. A., Kunin C. M. Quantitative urine culture method using a plastic "paddle" containing dual media. Appl Microbiol. 1972 May;23(5):919–922. doi: 10.1128/am.23.5.919-922.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Czerwinski A. W., Wilkerson R. G., Merrill J. A., Braden B., Colmore J. P. Further evaluation of the Griess test to detect significant bacteriuria. II. Am J Obstet Gynecol. 1971 Jul 1;110(5):677–681. doi: 10.1016/0002-9378(71)90253-5. [DOI] [PubMed] [Google Scholar]
  4. Dodge W. F., West E. F., Fras P. A., Travis L. B. Detection of bacteriuria in children. J Pediatr. 1969 Jan;74(1):107–110. doi: 10.1016/s0022-3476(69)80015-6. [DOI] [PubMed] [Google Scholar]
  5. Finnerty F. A., Jr, Johnson A. C. A simplified accurate method for detecting bacteriuria. Am J Obstet Gynecol. 1968 May 15;101(2):238–243. doi: 10.1016/0002-9378(68)90193-2. [DOI] [PubMed] [Google Scholar]
  6. Guttmann D., Naylor G. R. Dip-slide: an aid to quantitative urine culture in general practice. Br Med J. 1967 Aug 5;3(5561):343–345. doi: 10.1136/bmj.3.5561.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOEPRICH P. D. Culture of the urine. J Lab Clin Med. 1960 Dec;56:899–907. [PubMed] [Google Scholar]
  8. KAHLER R. L., GUZE L. B. Evaluation of the Griess nitrite test as a method for the recognition of urinary tract infection. J Lab Clin Med. 1957 Jun;49(6):934–937. [PubMed] [Google Scholar]
  9. KASS E. H. Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic. AMA Arch Intern Med. 1957 Nov;100(5):709–714. doi: 10.1001/archinte.1957.00260110025004. [DOI] [PubMed] [Google Scholar]
  10. Kunin C. M., Bergeron J. A. A simple quantitative urine culture method using an internally coated plastic pipette. Am J Clin Pathol. 1972 Oct;58(4):371–375. doi: 10.1093/ajcp/58.5.371. [DOI] [PubMed] [Google Scholar]
  11. Mackey J. P., Sandys G. H. Laboratory diagnosis of infections of the urinary tract in general practice by means of a dip-inoculum transport medium. Br Med J. 1965 Nov 27;2(5473):1286–1288. doi: 10.1136/bmj.2.5473.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matsaniotis N., Danelatou-Athanassiadou C., Katerelos C., Hartokalis P., Apostolopoulou E. Low urinary glucose concentration: a reliable index of urinary tract infection. J Pediatr. 1971 May;78(5):851–858. doi: 10.1016/s0022-3476(71)80358-x. [DOI] [PubMed] [Google Scholar]
  13. NETER E. EVALUATION OF THE TETRAZOLIUM TEST FOR THE DIAGNOSIS OF SIGNIFICANT BACTERIURIA. JAMA. 1965 May 31;192:769–769. doi: 10.1001/jama.1965.03080220033007. [DOI] [PubMed] [Google Scholar]
  14. Papanayiotou P., Papanayiotou K., Dontas A. S., Marketos S. G. Mass detection of bacteriuria by combination of two screening tests. Postgrad Med J. 1970 Jul;46(537):425–427. doi: 10.1136/pgmj.46.537.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Resnick B., Cella R. L., Soghikian K., Lieberman A. H., Weil E. Mass detection of significant bacteriuria. An improved triphenyltetrazolium chloride (TTC) technique. Arch Intern Med. 1969 Aug;124(2):165–169. [PubMed] [Google Scholar]
  16. SCHAUS R. Griess' nitrite test in diagnosis of urinary infection. J Am Med Assoc. 1956 Jun 9;161(6):528–529. doi: 10.1001/jama.1956.62970060009009d. [DOI] [PubMed] [Google Scholar]
  17. Scherstén B., Dahlqvist A., Fritz H., Köhler L., Westlund L. Screening for bacteriuria with a tet paper for glucose. JAMA. 1968 Apr 15;204(3):205–208. doi: 10.1001/jama.1968.03140160015004. [DOI] [PubMed] [Google Scholar]
  18. Walsh H., Hildebrandt R., Prystowsky H. A simplified technique for determining significant colony counts in urine. Am J Obstet Gynecol. 1966 Oct 15;96(4):585–587. doi: 10.1016/s0002-9378(16)34700-7. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES