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. 1973 Aug;26(2):206–210. doi: 10.1128/am.26.2.206-210.1973

Effects of Various Gases on the Survival of Dried Bacteria During Storage

Betty J Marshall 1,2,3, G G Coote 1,2,3, W J Scott 1,2,3
PMCID: PMC379752  PMID: 4200630

Abstract

Salmonella newport and Pseudomonas fluorescens were dried together in papain digest broth and sucrose-glutamate, and stored in several gases at various water activities (aw) between 0.00 and 0.40 at 25 C for various periods up to 81 weeks. Both S. newport and P. fluorescens, dried in papain digest broth and stored in air, died rapidly if the conditions were very dry (0.00 aw) or moist (0.40 aw). Storage in carbon dioxide and argon gave greater survival than storage in air but lower survival than did storage in nitrogen or in vacuo. When the organisms were dried in a sucrose-glutamate mixture the differences between the gases were very small, and variations in residual water were less important. Of the inert gases, argon gave the best survival when the organisms were dried in papain digest broth, especially at 0.00 aw; the survival in neon and krypton was lower and in xenon and helium it was much lower.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Greiff D., Rightsel W. A. Stabilities of dried suspensions of influenza virus sealed in a vacuum or under different gases. Appl Microbiol. 1969 Jun;17(6):830–835. doi: 10.1128/am.17.6.830-835.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HECKLY R. J. Preservation of bacteria by lyophilization. Adv Appl Microbiol. 1961;3:1–76. doi: 10.1016/s0065-2164(08)70506-9. [DOI] [PubMed] [Google Scholar]
  3. Naylor H. B., Smith P. A. Factors Affecting the Viability of Serratia marcescens During Dehydration and Storage. J Bacteriol. 1946 Nov;52(5):565–573. doi: 10.1128/jb.52.5.565-573.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. SCOTT W. J. The effect of residual water on the survival of dried bacteria during storage. J Gen Microbiol. 1958 Dec;19(3):624–633. doi: 10.1099/00221287-19-3-624. [DOI] [PubMed] [Google Scholar]

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