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Biophysical Journal logoLink to Biophysical Journal
. 2013 Oct 15;105(8):1822–1828. doi: 10.1016/j.bpj.2013.09.008

IR Laser-Induced Perturbations of the Voltage-Dependent Solute Carrier Protein SLC26a5

Oluwarotimi Okunade , Joseph Santos-Sacchi †,‡,§,
PMCID: PMC3797594  PMID: 24138858

Abstract

Alterations in membrane capacitance can arise from linear and nonlinear sources. For example, changes in membrane surface area or dielectric properties can modify capacitance linearly, whereas sensor residues of voltage-dependent proteins can modify capacitance nonlinearly. Here, we examined the effects of fast temperature jumps induced by an infrared (IR) laser in control and prestin (SLC26a5)-transfected human embryonic kidney (HEK) cells under whole-cell voltage clamp. Prestin’s voltage sensor imparts a characteristic bell-shaped, voltage-dependent nonlinear capacitance (NLC). Temperature jumps in control HEK cells cause a monophasic increase in membrane capacitance (Cm) regardless of holding voltage due to double-layer effects. Prestin-transfected HEK cells, however, additionally show a biphasic increase/decrease in Cm with a reversal potential corresponding to the voltage at peak NLC of prestin (Vh), attributable to a rapid temperature-following shift in Vh, with shift rates up to 14 V/s over the course of a 5 ms IR pulse. Treatment with salicylate, a known inhibitor of NLC, reestablishes control cell behavior. A simple kinetic model recapitulates our biophysical observations. These results verify a voltage-dependent protein’s ability to respond to fast temperature perturbations on a par with double-layer susceptibility. This likely arises from prestin’s unique ability to move sensor charge at kilohertz rates, which is required for the outer hair cells’ role as a cochlear amplifier.

Introduction

Outer hair cells (OHCs) are mechanically active components of the inner ear that underlie cochlear amplification (1). Cochlear amplification denotes a process whereby responses to low-level acoustic stimuli are enhanced, resulting in an increase in auditory sensitivity and frequency-resolving power. This is accomplished by OHCs feeding back mechanical energy into the vibrating sensory organ to boost stimulation to the inner hair cells, which are predominantly innervated by the auditory nerve. Prestin motor units (SLC26a5), proteins of the SLC26 anion transporter family (2), are localized to the OHC lateral membrane, drive rapid mechanical changes in OHCs, and are associated with nonlinear capacitance (NLC). NLC arises as the first derivative of a two-state Boltzmann function relating prestin voltage-sensor charge as a function of transmembrane voltage. It reflects the movement of charged residues within these motor units and peaks at a voltage (Vh) to which the OHCs’ mechanical response is maximally sensitive. NLC is vulnerable to biophysical forces, including temperature and membrane tension (3–8).

Here, we examine the effects of fast temperature jumps induced by an infrared (IR) laser in control and prestin-transfected human embryonic kidney (HEK) cells under whole-cell voltage clamp. We find effects on both linear and prestin-derived NLC. Whereas fast temperature jumps monotonically increase linear Cm in a voltage-independent manner, the Boltzmann distribution of motors along the voltage axis is rapidly and simultaneously altered in a reversible manner. Our observations clearly show that voltage-dependent proteins, given sufficiently fast kinetics (as with prestin), can contribute to rapid alterations of membrane capacitance.

Materials and Methods

Cell culture and expression

We previously developed a tetracycline-inducible HEK293 cell line that highly expresses prestin (9). Here, we cultured HEK293 cells in Dulbecco’s modified Eagle’s high glucose base medium (DMEM) containing 50 U/ml each of penicillin, streptomycin, and L-glutamine, supplemented with 10% fetal bovine serum, 5 μg/ml of blasticidin, and 130 μg/ml of zeocin. The cells were maintained at 37°C in a humidified incubator gassed with 5% CO2. The addition of 1 microgram/ml tetracycline to the growth medium induced prestin expression and trafficking to the cell membrane. Patch-clamp recordings of the cells were made 24–72 hr after induction at room temperature.

IR laser

Photonic stimulation with a Capella R-1850 laser was used to deliver rapid temperature jumps to cells in the recording chamber. The laser was coupled to a 600-μm-diameter optical fiber that delivered an output wavelength of 1850 nm in pulses of variable duration. At a power setting of 100%, the optical pulse energy was 5 mJ/ms. Laser stimulation was computer-controlled via TTL and synchronized to voltage clamp commands using an Axon Instruments 1320 series A/D and D/A board. The laser fiber was mounted on a micromanipulator and the tip was placed within 0.5 mm of the recorded cell, ensuring that the whole cell was irradiated.

We assume that our observations arise from temperature changes within the membrane fostered by temperature changes in bath solution water, in accord with conclusions made in previous studies (10,11). We calibrated the temperature change indirectly by monitoring changes in patch electrode resistance (Rs, i.e., changes in IRs with fixed voltage stimulation) as in the previous studies. Thus, in preliminary experiments, we correlated Rs versus changes in whole bath temperature. In the physiological experiments, our admittance analysis of currents allowed us to quantify Rs changes independently of Cm and Rm changes (12). We found that a 33% change in Rs indicated a temperature change of 17°C. In our experiment, peak Rs change averaged 31.2 ± 0.02% (n = 5) at 40% laser power. Our previous observation that a 20 mV shift in NLC Vh occurs per 10°C change in bath temperature corroborates these estimates (3,4).

Patch-clamp electrophysiology

Ionic blocking solutions were used to remove voltage-dependent ionic conductances so that capacitive currents could be analyzed in isolation. Extracellular bath solutions for whole-cell recording in HEK293 cells consisted of (mM) 20 TEA, 20 CsCl, 2 CoCl2, 1 MgCl2, 10 Hepes, 1 CaCl2, 100 NaCl adjusted to pH 7.22 with NaOH, and 301 mOsm using D-glucose. An extracellular perfusion solution containing 132 NaCl, 2 CaCl2, 2 MgCl2, 10 Hepes, 10 Na salicylate (pH 7.20, 300 mOsm) was also used for experiments to block NLC. Electrodes were filled with (mM) 140 CsCl, 2 MgCl2, 10 Hepes, 10 EGTA (pH 7.27, 302 mOsm). All chemicals used were purchased from Sigma.

Borosilicate glass pipettes were pulled using a P-2000 laser-heating pipette puller (Sutter Instruments) to initial resistances ranging between 3.5 and 5 MΩ. Pipette stray capacitance was compensated for before recordings were obtained, and voltages were corrected for effects of series resistance offline. A Nikon Eclipse TE300 inverted microscope with Hoffmann optics was used to observe the cells during electrical recording. Round, isolated cells growing on a glass coverslip were patched 24–72 hr after tetracycline induction.

Cells were clamped to a holding potential of 0 mV using an Axon 200B patch-clamp amplifier. During the temperature jump protocol, cells were held under voltage clamp at 0 mV and stepped in 30 mV increments (from hyperpolarizing values of −150 mV to 150 mV) for 1024 ms during which a brief IR laser pulse was delivered to the cells for each voltage step. Solution exchange (e.g., with salicylate) was performed using gravity flow. All recordings were made at room temperature. Local temperature measurements of the cells were calculated by measuring changes in electrode resistance and extrapolating from a resistance-temperature calibration curve (11). A resistance-temperature calibration curve was obtained by observing changes in pipette resistance in a hot bath solution (∼55°C) that was allowed to cool passively.

Cell capacitance was measured under a whole-cell configuration using jClamp software (SciSoft, CT; www.SciSoftCo.com). Voltage records sampled at 10 μs were filtered at 10 kHz with an eight-pole Bessel filter and saved to disk for offline analysis. NLC was measured using a continuous high-resolution (2.56 ms sampling), two-sine stimulus protocol (10 mV peak at both 390.6 and 781.2 Hz) superimposed onto the voltage command (12,13). Capacitance data were fit to the first derivative of a two-state Boltzmann function (14):

Cm=QmaxzekTb(1+b)2+Clin,b=exp(ze(VmVh)kT) (1)

where Qmax is the maximum nonlinear charge moved; Vh is voltage at peak capacitance or, equivalently, at half-maximum charge transfer; Vm is membrane potential; z is valence; Clin is linear membrane capacitance; e is electron charge; k is Boltzmann’s constant; and T is absolute temperature. Our justification for using steady-state fits of prestin’s charge movement at the 2.56 ms Cm measurement sampling rate is that the parameters are largely stable (z, Qmax), except for Vh, across time samples. ΔCm is defined as the difference between pre-IR and post-IR maximal capacitance. Results are reported as the mean ± standard error (SE).

Model

To understand the biophysical data, we used a kinetic model of SLC26a5 that we previously developed (15). The model was developed to account for a chloride-dependent disparity between NLC and electromotility Vh, requiring intermediate transitions much slower than either chloride- or voltage-dependent transitions. A model cartoon is shown in Fig. 5 A of Song and Santos-Sacchi (15).

Xok2k1Xcβ0α0XβαC (2)

State X0 is SLC26a5 in the absence of bound anion; state Xc is with bound anion; state X is voltage-enabled after anion binding, with its residence being favored by hyperpolarization (expanded state); and state C results from depolarization (contracted state). The model has very fast anion (chloride) binding and unbinding transitions (XoXc) and very fast voltage-dependent conformational transitions (XC). The transition to state C carries a unit charge, q; Qmax, the total charge moved, will reflect the maximal accumulation of motors in that state. Nestled between the anion-binding and voltage-dependent transitions resides a nonvoltage-dependent transition (XcX), which is very much slower than the other two transitions.

We assessed the kinetic model using MATLAB (The MathWorks, Natick, MA) Simulink via an automation link with jClamp (www.scisoftco.com). The kinetic model was interfaced to jClamp via a model of the patch-clamp amplifier and cell. The linear component of the patch-cell model possessed an Rs of 5 MΩ, Rm of 500 MΩ, and Clin of 10 pF. The nonlinear component, NLC, derived from charge movement of the SLC26a5 model, with the following parameters: k1 = 107 [Cl]in, k2 = 107 kd, α0 = 75 exp(tm), β0 = 95, α = 106 exp(zFVm/2RT), β = 583 109 exp(−zFVm/2RT + tm/2 – Ea/RT), kd Cl = 0.001,T = 296 kelvin, Ea = 45 kJ/mol, F = 9.648 104 C/mol, R = 8.315 J/Kmol, k = 1.381 10−23 VC/K.

The rates α0 and β are tension sensitive because tension is known to increase prestin residence in the expanded (X) state (5,6), tm in units kT (eRT/F); the rate β is temperature sensitive, with Arrhenius activation energy, Ea. α and β are voltage dependent, with sensor valence z = 0.7. At T = 296 K, Vm = 0 mV, Tm = 0 kT, and [Cl]in = 0.14 M, initial rates are k1 = 1.4e6, k2 = 1e4, α0 = 75, β0 = 95, α = 1e6, and β = 1e5. Chloride concentrations and Kd are in molar; rates are 1/t, with time (t) being 1 s. Voltage is in volts.

Results

Capacitance

The rapid temperature change associated with an IR laser pulse delivered directly to a cell via optical fiber alters membrane capacitance. Control HEK cells and cells of our uninduced SLC26a5 HEK cell line increase their linear membrane capacitance in a voltage-independent fashion. Fig. 1 A shows Cm measures of a voltage-clamped, uninduced SLC26a5 HEK cell for a wide range of holding potentials. The delivery of a 20 ms IR pulse (40% of maximal laser power) induces a linearly ramped increase in Cm coinciding with the duration of the pulse. To better visualize the overlapped traces and indicate holding voltage, we offset the traces by an arbitrary constant, allowing clearer observation of the voltage independence. The increase in Cm is 10.8% ± 2.5% (n = 5) of whole-cell capacitance for a 20 ms pulse. In Fig. 1 A, at laser offset, a single exponential decrease in Cm occurs with a time constant of 70 ms at 0 holding potential (81.5 ± 3.2 ms; n = 5). These linear and exponential phases of Cm change correspond, respectively, to a linear increase in temperature during the pulse and an exponential cooling of the bath solution/cytoplasm after the pulse, both of which are reflected in simultaneous changes in the series resistance of the pipette electrode (Fig. 1 B). Our admittance analysis allows us to quantify Rs changes, which are known to correspond to temperature manipulations (11). Fig. 1 C shows that increases in pulse durations induce increasing temperature changes that evoke larger Cm responses. Within our exposure range, we do not see any threshold effect for cell damage that is not associated with loss of cell recording.

Figure 1.

Figure 1

IR laser-induced temperature jump alters linear capacitance. (A) Under whole-cell voltage clamp, an uninduced SLC26a5 HEK cell was nominally stepped to the membrane potentials indicated. During the voltage step, an IR laser pulse of 20 ms duration (nominally 40% Capella laser power) was delivered via optical fiber. Regardless of the holding potential, the laser pulse induced a fixed maximal increase in Cm, ∼10% of resting Cm. Averages are given in Results section. (B) Simultaneously measured series resistance indicates a linear increase in temperature during the pulse and an exponential cooling of bath media after the pulse. (C) An increase in duration of the pulse results in a greater Cm change. The holding potential is 0 mV.

In our SLC26a5 HEK cell line after tetracycline induction, cells possess a voltage-dependent NLC atop their linear capacitance (16). This arises from the voltage sensor activity underlying the protein’s role in OHC electromotility (3,4). Fig. 2 illustrates the voltage-dependent nature of the induced HEK cell’s Cm, and the influence of temperature jumps on NLC and linear Cm. Again, we offset the overlapped traces by an arbitrary constant, allowing clearer observation of the effects of IR pulse on Cm; obvious differences are found in comparison with uninduced HEK cells (see Fig. 1). Indeed, a voltage-dependent effect is now observed. In Fig. 2 B, Cm-Vm functions are plotted at various time points relative to the start of the IR pulse. The laser pulse induced a shift of the Cm-Vm relation in the depolarizing direction. After correction of voltages for Rs effects, Boltzmann fits to the data (see Materials and Methods) allow a high-resolution (2.56 ms) inspection of dynamic changes in NLC and linear capacitance during and after the IR pulse (Fig. 2 C). In this example, NLC Vh shifted 40 mV in 20 ms at a linear rate of 2.03 V/s (average is 2.32 ± 0.21 V/s; n = 6) during the heating phase, and recovers (with temperature) exponentially with a time constant of 73 ms (average is 65.4 ± 10.8 ms; n = 6) during the cooling phase. The shift in Vh represents a redistribution of prestin motors into the expanded conformation during heating. We previously observed this shift over the course of minutes using Peltier control of the bath solution temperature, with the shift averaging ∼20 mV/10°C (3,4). In two additional cells, we were able to determine the Vh shift with 5 ms pulses at 90% laser power. The shift was 67 and 70 mV in 5 ms or 13.4 and 14 V/s, indicating that heating rates and corresponding Vh shift rates increase with greater laser power. The increase in rates with laser power indicates that we have yet to observe the fastest response and are limited by the laser power (for technical reasons, we avoid >90% power usage). Linear capacitance also changes simultaneously, with a time course similar to that of NLC Vh. In this case, there is a linear change of 50 pF/s during heating and a recovery due to cooling with a time constant of 69 ms (average is 78.9 ± 7.7 ms; n = 6). The changes due to cooling in linear capacitance are similar to those observed in control HEK cells. These rapid rates of change during heating and cooling mirror the changes in temperature as gauged from Rs inspection or predicted from previous observations on temperature-dependent shifts of Vh during slow bath changes in temperature (3,4), i.e., they correspond to a temperature-induced change of 20 mV/10°C.

Figure 2.

Figure 2

IR laser-induced temperature jump alters SLC26a5-generated NLC and linear capacitance. (A) Under whole-cell voltage clamp, an induced SLC26a5 HEK cell was nominally stepped to the membrane potentials indicated. During the voltage step, an IR laser pulse of 20 ms duration (nominally 40% Capella laser power) was delivered via optical fiber. The laser pulse induced a maximal change in Cm that depended on the holding potential. The change could be either an increase or decrease. (B) Cm-Vm plots of NLC as a function of time after pulse onset. Note the effect on the voltage dependence of NLC, namely, a shift to the right. (C) Changes in Vh and Clin follow temperature. Rapid shifts and increases in Clin occur during laser heating and return back to initial levels during bath cooling. Averages are given in the Results section. To see this figure in color, go online.

The difference in susceptibility of NLC and linear Cm to temperature jump is readily illustrated by the behavior of ΔCm, defined as the maximal difference between pre-IR and post-IR capacitance. Examples from two cells are shown in Fig. 3, A and B. Whereas IR pulse-induced linear Cm changes occur at the same magnitude and direction (increase) regardless of the holding potential (Fig. 1), NLC changes vary depending on the holding potential, and reverse in direction near voltages (average is −96.8 ± 6.4 mV; n = 5) around NLC Vh (average is −94.7 ± 6.2 mV; n = 5), with an R2 value of 0.9943 (Fig. 3, A and B). Thus, it is possible, depending on the magnitude of NLC and its Vh (relative to holding potential), to induce a decrease in Cm by IR laser pulse. Salicylate (10 mM) not only reduces NLC, as expected (18,19), but also eliminates the characteristic reversal of ΔCm normally afforded by SLC26a5 expression, essentially returning the induced HEK cell back to its preinduced condition (n = 2). That is, only an increase in Cm is observed, regardless of the holding potential (Fig. 3 A).

Figure 3.

Figure 3

IR laser-induced temperature jump alters NLC showing increases and decreases that reverse near Vh of NLC. The NLC plotted is the one before the temperature jump. (A and B) Shown are data from two cells. ΔCm at positive voltages remains offset from zero due to the temperature-dependent increase in linear Cm (curly brackets). In the first case (A), after data collection, salicylate (10 mM) was perfused onto the cell and collection was repeated. Salicylate removes the ΔCm reversal as a result of NLC block, leaving intact a constant linear Cm increase across holding voltage. Averages are given in Results.

To understand the data, we evaluated the temperature-dependent behavior of a recently developed kinetic model of SLC26a5 (15). In this model (see Materials and Methods), only the backward, voltage-dependent transition, β, is temperature sensitive, indicating that only movements into the hyperpolarized (expanded) state of SCL26a5 are affected by temperature. In the simulation, we simply modeled the temperature change as that revealed by our experimental measures of Rs (in this case, with a 23°C maximum change; Fig. 4 A). Similar to the biophysical data, a rapid temperature change followed by cooling induced characteristic changes in Cm, which derived from NLC magnitude and induced Vh shifts (Fig. 4, B and C). As we deduced from the biophysical data, NLC Vh shifts directly mirror temperature changes. To match the average biophysical data of 2.3V/s (∼20 mV/10°C), an Arrhenius activation energy of 45 k J/mol was required. The model also recapitulates the reversal of ΔCm near Vh (Fig. 4 D). Also note that ΔCm recovers with temperature back to zero at voltages away from Vh, in contrast to the biophysical data (Fig. 3), because the original model had no temperature-sensitive linear Cm (Fig. 4, solid circles). However, when a linearly temperature-dependent Cm is introduced, ΔCm appears more similar to the biophysical data (Fig. 4 D, open circles). The original implementation of the kinetic model (15) had temperature dependence of both the backward intermediate rate, β0, and the backward voltage-dependent rate, β. Here, however, we obtained better correspondence to the biophysical data by setting temperature dependence only in β.

Figure 4.

Figure 4

A kinetic model recapitulates biophysical data. (A) Stimulation with the experimentally observed temperature change induced by an IR laser pulse induces Cm behavior similar to the biophysical results. Here, the model transition rates are set to give a Vh of −71 mV. (B) Plots of NLC show rightward shifts along the voltage axis as a result of temperature increase. (C) Rates of Vh shift are comparable to biophysical data and directly follow temperature alterations. (D) Voltage at reversal of ΔCm is related to Vh. Note that ΔCm goes back to zero, in contrast to the biophysical data, when Clin is not temperature sensitive (closed circles). Including temperature dependence for Clin results in an offset similar to the biophysical data (open circles, curly brackets). To see this figure in color, go online.

Currents

We found two components of currents associated with fast temperature jump (Fig. 5). The first component coincided with the IR heating phase and its magnitude was related to the rate of heating (or correspondingly to the rate of linear Cm change; Fig. 5, A and B). This current appeared to reverse at positive voltages, as found by Shapiro et al. (10) (Fig. 5 C). We agree with their discussion on the matter, especially their interpretation that this may arise from asymmetrical fixed charges on the membrane leaflets. The second, slower component, which reversed near 0 mV, peaked at maximal temperature and then decayed during the cooling phase (Fig. 5, C and D). We interpret this as an ionic current that is nonspecific with a reversal potential near zero. Sometimes we found that the second, slower component obscured the first component as described in Shapiro et al. (10). Nevertheless, the rapid component is clearly observable in the traces near zero potential (Fig. 5 C). The second, slow-decaying membrane current displays nonlinear features (Fig. 5 D) reminiscent of GmetL, a conductance found in OHCs with marked temperature dependence (20,21). The current increase due to IR stimulation is likewise nonlinear, and therefore is unlikely due to linear decreases in Rs with temperature.

Figure 5.

Figure 5

Two components of current induced during temperature jump. (A and B) Capacitance increases during temporally incremental IR laser pulses (A) and corresponding currents arise (B). Current magnitude depends on rate of Cm change and duration depends on pulse duration. For all traces, pulses were delivered at a holding potential of 0 mV. (C) Current responses for a 20 ms duration pulse within the holding potential extremes indicated, stepped nominally at 30 mV. Note the fast inward current response during the laser pulse, with a reversal potential predicted to be at positive potentials. Simultaneously, a slow-decaying current with reversal near 0 mV is observed. The red arrow shows the sum of the two components (note step behavior around zero potential that rides on the slower current ramp) and the blue arrow shows the slow component after the fast component ends after the laser pulse. (D) IV plot of average cell currents before and at termination of the IR pulse (mean ± SE; n = 5). The red line is IR-induced current. Note that current voltage dependence is unaffected by temperature, unlike that of NLC.

Discussion

Control HEK cells and HEK cells expressing the voltage-dependent protein SLC26a5 show very fast changes in membrane capacitance when rapid temperature jumps are delivered to the cells by IR laser. For control cells, rapid alterations in linear Cm directly follow changes in temperature, as observed by Shapiro et al. (10). With the pulse energies we used, the rates of change were up to 50 pF/s and magnitudes peaked near 10% of the cell’s resting membrane capacitance. Effects were reversible upon heat dissipation through the bathing solution. HEK cells expressing prestin showed additional effects on the Boltzmann characteristics of voltage sensor-related NLC. With 20 ms IR pulse durations, Vh was shifted in the positive direction at an average rate of 2.3 V/s, corresponding to 20 mV/°C, and subsequently returned to prior conditions as the heat dissipated. In two cells, delivery of near-maximum laser power produced Vh shift rates of 13–14 V/s. The rates of recovery of linear Cm and Vh with cooling were indistinguishable, indicating that each is directly coupled to thermal energy. A simple kinetic model of prestin (15) that possesses temperature-sensitive, voltage-dependent transitions that move prestin into the expanded state can mimic our biophysical observations. Thus, we conclude that temperature-induced alteration of transition rates between voltage-dependent conformations of prestin can alter NLC as fast as thermal influences on the membrane double layer (10).

Capacitance

It is well established that capacitance is temperature sensitive; for example, Taylor (22) reported that squid axon capacitance increased 1% per degree centigrade. Others have reported that the kinetics of voltage-dependent channels is temperature sensitive, consequently influencing gating currents (23,24) and necessarily altering their equivalent voltage-dependent capacitance. It is therefore not surprising that prestin NLC is temperature dependent, as we previously showed with slow alterations in bath temperature (3,4). Prestin’s NLC Vh shifts ∼20 mV per 10°C. What may appear surprising is that prestin can redistribute between expanded and contracted states so rapidly upon IR stimulation, showing Vh shift rates up to 14 V/s. Of course, by nature, prestin is poised to transition between states at kilohertz rates, and apparently is capable of rapidly responding to a variety of energy forms, including mechanical, electrical, and thermal forms (7,8,25,26). Nevertheless, our observations are not simply related to the ability of prestin to rapidly transition between states; more importantly, they reflect the efficacy of realigning the protein’s operating voltage range by external influences, namely, adjusting the gain of cochlear amplification.

Our modeling of temperature effects on prestin’s voltage-dependent backward rate is similar to that employed to understand TRP channel heat sensitivity (27). In this regard, we recently found that prestin is sensitive to capsaicin (28), an agent that mimics heat activation of TRP channels. However, in prestin, the mechanism of action must be different from that of temperature since it causes negative shifts in Vh, pointing toward action on forward rates. Thus, it will be of interest to evaluate the simultaneous effects of capsaicin and temperature on prestin NLC. Of course, other proposed mechanisms in TRP channels that may also contribute to temperature sensing by prestin include allosteric mechanisms (29) and changes in specific heat capacity (30). Nevertheless, the simplest scheme is likely that of temperature-sensitive transition rates as modeled here, which show excellent agreement with the biophysical data. This is not to say that the sensitivities of the other transitions in the model, i.e., those displaying tension and chloride sensitivity, cannot influence the temperature-sensitive, voltage-dependent transition, as it is well known that a voltage-independent transition can affect the characteristics of a linked voltage-dependent one (31–33).

Currents

In this study, we used blockers to reduce ionic conductances so that we could effectively measure capacitance. We identified two components of residual current: one that is fast, coinciding with the duration of laser heating, and one that follows the temperature of the bath as it cooled. This observation is similar to that of Parker (24), who suggested that the fast component represents gating currents of oocyte ion channels. This was suggested because in the absence of the slow component (i.e., at its reversal potential), transient capacitive-like currents were revealed. Although we do expect that sensor charge movement (i.e., gating-like currents) should arise when prestin’s Vh suddenly shifts, we find no evidence of transient-like currents near the reversal potential of our slow component, around 0 mV. We should note that when the cell is held at 0 mV, we are interrogating a linear region of Cm, since NLC Vh is very negative. Our laser-induced currents at 0 mV resemble those found by Shapiro et al. (10), and we conclude that they represent currents generated by rapid changes in linear Cm. The second component of current, which reverses near zero, has some nonlinear features of a conductance, GmetL, found in OHCs (20,21). Notably, increases in temperature augment the nonlinear current and are not simply leakage currents.

In summary, we find that SLC26a5 (prestin) is remarkably responsive to fast temperature jumps, rapidly moving its operating point along the voltage axis. This susceptibility to thermal perturbations likely arises from the protein’s natural ability to follow voltage changes at acoustic frequencies, but it also has implications for manipulation of cochlear amplifier gain control. Thus, we predict that we may be able to drive auditory sensation by stimulating OHCs with a high-frequency gated IR laser, and manipulate cochlear amplification in vivo.

Acknowledgments

The authors thank Lei Song and Jun-Ping Bai for their technical assistance and discussions. This research was supported by NIH NIDCD grant DC00273 to J.S.S. and an HHHMI Medical Research Fellowship to O.O.

Footnotes

This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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