Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1973 Sep;26(3):258–263. doi: 10.1128/am.26.3.258-263.1973

System for Studying Uninfected and Virus-Infected Cell Cultures in Hyperbaric Chambers1

J K L Leong a, W W Schultz a, E Zebovitz a, N A Schlamm a
PMCID: PMC379770  PMID: 4796161

Abstract

Equipment and techniques previously used to investigate the effect of hyperbaric gases on bacteria were modified to permit comparable investigations with uninfected and virus-infected tissue cell cultures. This report describes the modified equipment and related methodology. Use of the system is illustrated with findings on the effect of oxygen-helium mixtures at 68 atm on cell physiology and virus growth in two cell types. Our results suggested that, under those experimental conditions, several synthetic processes in chick fibroblast monolayers are inhibited but that Sindbis virus growth in the cells is increased. Growth of Japanese encephalitis virus in porcine kidney cells was found to be unaffected by oxygen-helium gas at partial pressures of oxygen between 0 and 700 mm Hg, but morphological alterations in the cells occurred at low and high pO2 levels.

Full text

PDF
258

Images in this article

262Image
on p.262
262Image
on p.262

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Daily O. P., Schlamm N. A. Effect of hyperbaric atmospheres on -galactoside transport in Escherichia coli. Can J Microbiol. 1972 Jul;18(7):1162–1164. doi: 10.1139/m72-179. [DOI] [PubMed] [Google Scholar]
  2. Inoue Y. K., Yamada M. A. Clonal line of porcine kidney stable cells for assay of Japanese encephalitis virus. J Bacteriol. 1964 May;87(5):1239–1240. doi: 10.1128/jb.87.5.1239-1240.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. SEGRE D. CYTOPATHIC EFFECTS OF NORMALLY NON-CYTOPATHOGENIC VIRUSES IN TISSUE CULTURES HELD UNDER INCREASED OXYGEN PRESSURE. Proc Soc Exp Biol Med. 1964 Nov;117:567–569. doi: 10.3181/00379727-117-29639. [DOI] [PubMed] [Google Scholar]
  4. Schlamm N. A., Coykendall A. L., Meyers C. E. Effect of penicillin on Staphylococcus aureus cultivated at high atmospheric pressure. J Bacteriol. 1969 Apr;98(1):327–328. doi: 10.1128/jb.98.1.327-328.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schlamm N. A., Daily O. P. Effect of elevated atmospheric pressure on penicillin binding by Staphylococcus aureus and Streptococcus pyogenes. Antimicrob Agents Chemother. 1973 Feb;3(2):147–151. doi: 10.1128/aac.3.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Schlamm N. A., Daily O. P. Uptake of beta-galactosides by Escherichia coli at elevated atmospheric pressure. J Bacteriol. 1971 Mar;105(3):1202–1204. doi: 10.1128/jb.105.3.1202-1204.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schlamm N. A. Effect of elevated atmospheric pressure on antibiotic susceptibility of Staphylococcus aureus and Streptococcus pyogenes. Antimicrob Agents Chemother. 1972 Jun;1(6):512–513. doi: 10.1128/aac.1.6.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Taylor G. W., Kondig J. P., Nagle S. C., Jr, Higuchi K. Growth and metabolism of L cells in a chemically defined medium in a controlled environment culture system. I. Effects of O2 tension on L-cell cultures. Appl Microbiol. 1971 May;21(5):928–933. doi: 10.1128/am.21.5.928-933.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Zebovitz E., Leong J. K., Doughty S. C. Japanese encephalitis virus replication: a procedure for the selective isolation and characterization of viral RNA species. Arch Gesamte Virusforsch. 1972;38(4):319–327. doi: 10.1007/BF01262822. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES