Abstract
A group of 277 yeasts isolated from burned children and 14 reference strains were tested for phosphatase activity by using phenolphthalein phosphate substrates. Phosphatase activity was widely distributed among various species and strains representing seven genera. Candida albicans, which was the most common yeast isolated from clinical material, was notably absent in producing the enzyme, whereas Candida tropicalis was the most consistent, strong, and rapidly active phosphatase-producing organism. The characteristic enzyme activity of a selected isolate of C. tropicalis was demonstrated in the presence of concentrations of inorganic phosphate which inhibited enzyme activity of other species. The greater enzyme activity of C. tropicalis was not related to more rapid or greater cell growth or decrease in the pH of culture media. Extracellular constitutive heat-labile acid phosphatase was found in broth filtrates of C. tropicalis, C. krusei, and a strain of Staphylococcus aureus.
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- BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
- BARBER M., KUPER S. W. A. Identification of Staphylococcus pyogenes by the phosphatase reaction. J Pathol Bacteriol. 1951 Jan;63(1):65–68. doi: 10.1002/path.1700630108. [DOI] [PubMed] [Google Scholar]
- Bayliss M., Glick D., Siem R. A. Demonstration of Phosphatases and Lipase in Bacteria and True Fungi by Staining Methods and the Effect of Penicillin on Phosphatase Activity. J Bacteriol. 1948 Mar;55(3):307–316. doi: 10.1128/jb.55.3.307-316.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chattaway F. W., Odds F. C., Barlow A. J. An examination of the production of hydrolytic enzymes and toxins by pathogenic strains of Candida albicans. J Gen Microbiol. 1971 Aug;67(3):255–263. doi: 10.1099/00221287-67-3-255. [DOI] [PubMed] [Google Scholar]
- Dolan C. T., Ihrke D. M. Further studies of the germ-tube test for Candida albicans identification. Am J Clin Pathol. 1971 Jun;55(6):733–734. doi: 10.1093/ajcp/55.6.733. [DOI] [PubMed] [Google Scholar]
- Montes L. F., Wilborn W. H. Acid phosphatase activity during phases of growth in Candida albicans. Int J Dermatol. 1970 Jul-Sep;9(3):220–225. doi: 10.1111/j.1365-4362.1970.tb05120.x. [DOI] [PubMed] [Google Scholar]
- Painter B. G., Isenberg H. D. Isolation of Candida parapsilosis: report of two cases. Am J Clin Pathol. 1973 Jan;59(1):62–65. doi: 10.1093/ajcp/59.1.62. [DOI] [PubMed] [Google Scholar]
- Smith R. F., Bettge C. L., Dayton S. L., Jorgensen J. H. Characterization of Staphylococcus aureus in a pediatric burn unit. Appl Microbiol. 1973 Jan;25(1):15–20. doi: 10.1128/am.25.1.15-20.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. F., Dayton S. L. Use of acetamide broth in the isolation of Pseudomonas aeruginosa from rectal swabs. Appl Microbiol. 1972 Jul;24(1):143–145. doi: 10.1128/am.24.1.143-145.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]