Abstract
Several strains of Mycobacterium and Nocardia were stained for acid fastness by using the method of Kinyoun. Duplicate, glutaraldehyde-fixed smears were extracted with fresh pyridine prior to staining. The results clearly demonstrated that both in vivo- and in vitro-grown Nocardia differed from Mycobacterium by becoming nonacid fast after extraction. This pyridine extraction of nocardial acid fastness should have diagnostic and taxonomic application.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradley S. G. Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation. J Bacteriol. 1973 Feb;113(2):645–651. doi: 10.1128/jb.113.2.645-651.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Convit J., Pinardi M. E. A simple method for the differentiation of Mycobacterium leprae from other mycobacteria through routine staining technics. Int J Lepr Other Mycobact Dis. 1972 Apr-Jun;40(2):130–132. [PubMed] [Google Scholar]
- Fisher C. A., Barksdale L. Cytochemical reactions of human leprosy bacilli and mycobacteria: ultrastructural implications. J Bacteriol. 1973 Mar;113(3):1389–1399. doi: 10.1128/jb.113.3.1389-1399.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fisher C. A., Barksdale L. Elimination of the acid fastness but not the gram positivity of leprosy bacilli after extraction with pyridine. J Bacteriol. 1971 May;106(2):707–708. doi: 10.1128/jb.106.2.707-708.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GEORG L. K., AJELLO L., McDURMONT C., HOSTY T. S. The identification of Nocardia asteroides and Nocardia brasiliensis. Am Rev Respir Dis. 1961 Sep;84:337–347. doi: 10.1164/arrd.1961.84.3.337. [DOI] [PubMed] [Google Scholar]
- GORDON R. E., MIHM J. M. A comparison of Nocardia asteroides and Nocardia brasiliensis. J Gen Microbiol. 1959 Feb;20(1):129–135. doi: 10.1099/00221287-20-1-129. [DOI] [PubMed] [Google Scholar]
- GORDON R. E., MIHM J. M. The type species of the genus Nocardia. J Gen Microbiol. 1962 Jan;27:1–10. doi: 10.1099/00221287-27-1-1. [DOI] [PubMed] [Google Scholar]
- LANGEVIN R. W., KATZ S. FULMINATING PULMONARY NOCARDIOSIS. Dis Chest. 1964 Sep;46:310–316. doi: 10.1378/chest.46.3.310. [DOI] [PubMed] [Google Scholar]
- Lechevalier M. P., Horan A. C., Lechevalier H. Lipid composition in the classification of nocardiae and mycobacteria. J Bacteriol. 1971 Jan;105(1):313–318. doi: 10.1128/jb.105.1.313-318.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neu H. C., Silva M., Hazen E., Rosenheim S. H. Necrotizing nocardial pneumonitis. Ann Intern Med. 1967 Feb;66(2):274–284. doi: 10.7326/0003-4819-66-2-274. [DOI] [PubMed] [Google Scholar]
- Ridell M., Norlin M. Serological study of nocardia by using mycobacterial precipitation reference systems. J Bacteriol. 1973 Jan;113(1):1–7. doi: 10.1128/jb.113.1.1-7.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]