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. 1973 Oct;26(4):478–484. doi: 10.1128/am.26.4.478-484.1973

Microtiter Solid-Phase Radioimmunoassay for Hepatitis B Antigen

Robert H Purcell 1,2, Doris C Wong 1,2, Harvey J Alter 1,2, Paul V Holland 1,2
PMCID: PMC379831  PMID: 4201649

Abstract

A micro-solid-phase radioimmunoassay (micro-SPRIA) for hepatitis B antigen (HB Ag) was developed for use with microtiter serological equipment. Radiolabeled immunoglobulin G was prepared from human and animal sera containing hepatitis B antibody (HB Ab); it was not necessary to isolate specific HB Ab by immunochemical means. A micro-SPRIA prepared with guinea pig reagents was approximately as sensitive as the AusRIA radioimmunoassay, but, like the AusRIA test, yielded false positive results. A micro-SPRIA prepared with human reagents was slightly less sensitive but did not yield false positive results. These micro-SPRIA tests offer several advantages, including conservation of reagents, adaptability to other antigen-antibody systems, ease of performance (especially when testing large numbers of specimens), and economy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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