Abstract
Lyophilized cells of Bacillus popilliae were protected from moisture when suspended in pellets of tung oil polymer which were then coated with paraffin wax. The survival of the protected cells at various levels of relative humidity (RH) and under various storage conditions was determined. During 6 months of storage, moisture appeared to have little effect on survival of the cells when the RH level was 22% or less; but, at higher RH levels, survival declined upon storage. Viable cells were recovered when pellets were stored for 3 months at 33% RH, 2 months at 42% RH, 1 month at 50% RH, and 4 days in distilled water. Under field conditions, some cells survived at least 1 week of storage.
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Selected References
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- Lingg A. J., McMahon K. J., Herzmann C. Viability of Bacillus popilliae after lyophilization of liquid nitrogen frozen cells. Appl Microbiol. 1967 Jan;15(1):163–165. doi: 10.1128/am.15.1.163-165.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lingg A. J., McMahon K. J. Survival of lyophilized Bacillus popilliae in soil. Appl Microbiol. 1969 May;17(5):718–720. doi: 10.1128/am.17.5.718-720.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ngel J. G., Kunz L. J. Simplified storage and retrieval of stock cultures. Appl Microbiol. 1972 Apr;23(4):837–838. doi: 10.1128/am.23.4.837-838.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]