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. 1973 Nov;26(5):744–746. doi: 10.1128/am.26.5.744-746.1973

Incorporation of Radioactive Acetate into Diacetyl by Streptococcus diacetilactis

R A Speckman 1, E B Collins 1
PMCID: PMC379894  PMID: 4148640

Abstract

Streptococcus diacetilactis was grown in a partially defined, lipoic acid-free medium containing radioactive acetate with and without addition of 0.1% unlabeled sodium pyruvate. Labeled carbon was incorporated into diacetyl, but neither the amount of diacetyl produced nor its specific activity was influenced by addition of pyruvate. Acetoin had low specific activity, indicating that it was a mixture of radioactive and nonradioactive acetoin. The specific activity of acetoin was lower when pyruvate, a precursor of unlabeled acetoin, was added to the medium, which indicated that the radioactive acetoin was produced from radioactive diacetyl by diacetyl reductase. Results substantiate condensation of acetyl-coenzyme A with hydroxyethylthiamine pyrophosphate as the in vivo mechanism for synthesis of diacetyl.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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