Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1973 Nov;26(5):751–756. doi: 10.1128/am.26.5.751-756.1973

Evaluation of a Fluorescent Antibody-Enrichment Serology Combination Procedure for the Detection of Salmonellae in Condiments, Food Products, Food By-Products, and Animal Feeds1

John S Hilker a,2, Myron Solberg a
PMCID: PMC379896  PMID: 4586931

Abstract

The reliability of the enrichment serology (ES), fluorescent antibody (FA), and a combination of the FA and ES procedures for the detection of salmonellae were compared to the Salmonella cultural procedure outlined in the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM). A total of 126 subsamples from 22 different products were analyzed. By utilizing the BAM procedure as the reference standard, a total of 66 samples were positive for salmonellae. Within 44 h approximately 65% of the Salmonella-negative samples could be cleared by the FA test. At the end of 50 h 97% of the Salmonella-negative samples could be cleared by the combination FA-ES test. The FA procedure detected all 66 BAM positives but exhibited a high incidence of presumptive positives which were cultural negatives. The ES procedure detected 64 of the 66 BAM positives but exhibited a low incidence of presumptive positives which were cultural negatives. Incorporating positive FA and positive ES results in a combination FA-ES technique revealed that FA-ES positives were statistically equivalent to BAM positives.

Full text

PDF
751

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banwart G. J., Kreitzer M. J. Rapid determination of Salmonella in samples of egg noodles, cake mixes, and candies. Appl Microbiol. 1969 Nov;18(5):838–842. doi: 10.1128/am.18.5.838-842.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EDWARDS P. R., FIFE M. A. Lysine-iron agar in the detection of Arizona cultures. Appl Microbiol. 1961 Nov;9:478–480. doi: 10.1128/am.9.6.478-480.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fantasia L. D., Sperber W. H., Deibel R. H. Comparison of two procedures for detection of Salmonella in food, feed, and pharmaceutical products. Appl Microbiol. 1969 Apr;17(4):540–541. doi: 10.1128/am.17.4.540-541.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
  6. Harrington R., Jr, Ellis E. M., Mallinson E. T., Ranck M., Solee R. E. An evaluation of a fluorescent antibody technique for the detection of Salmonellae in animal by-products, feeds, and tissues. J Am Vet Med Assoc. 1970 Dec 1;157(11):1898–1900. [PubMed] [Google Scholar]
  7. NORTH W. R., Jr Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products. J Bacteriol. 1960 Dec;80:861–861. doi: 10.1128/jb.80.6.861-861.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reamer R. H., Hargrove R. E., McDonough F. E. Increased sensitivity of immunofluorescent assay for Salmonella in nonfat dry milk. Appl Microbiol. 1969 Sep;18(3):328–331. doi: 10.1128/am.18.3.328-331.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reamer R. H., Hargrove R. E. Twenty-four-hour immunofluorescence technique for the detection of salmonellae in nonfat dry milk. Appl Microbiol. 1972 Jan;23(1):78–81. doi: 10.1128/am.23.1.78-81.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thomason B. M. Rapid detection of Salmonella microcolonies by fluorescent antibody. Appl Microbiol. 1971 Dec;22(6):1064–1069. doi: 10.1128/am.22.6.1064-1069.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES