Table 2.
Technique | Excitatin light source | Excitation wave- length selection |
Emission wavelength selection | Detection |
---|---|---|---|---|
Fluorometry (PTI Delta RAM) | Xe continuous 300–600 nm | Monochromator | Monochromator | Photomultiplier |
Fluorescence imaging (custom designed) | Xe continuous | Monochromator (PTI) Excitation filters (Lambda DG, Sutter or filter wheel, ASI) | Emission filters (filter wheel) | CCD camera (PXL, Photometrics or Pluto, Pixelvision) |
Confocal imaging (BioRad Radiance 2002) | Laser: 442 nm HeCd, 488 nm KrAr, 568 nm KrAr and 635 nm red diode | na | Emission filters (filter wheel) | Photomultiplier |