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. 1974 Jan;27(1):206–209. doi: 10.1128/am.27.1.206-209.1974

L-Asparaginase Production by the Rumen Anaerobe Vibrio succinogenes

David Kafkewitz 1, David Goodman 1
PMCID: PMC379995  PMID: 4855647

Abstract

The rumen anaerobe Vibrio succinogenes possesses a constitutive L-asparaginase. The amount of enzyme produced is affected by the compound supplied to the organism to generate the fumaric acid it requires as a terminal electron acceptor. When nitrate is provided as the terminal electron acceptor, the amount of enzyme produced is affected by the compound provided to satisfy the nutritional requirement of the organism for succinic acid. Specific activities of up to 8.4 IU/mg of protein in cell-free extracts have been obtained. This specific activity is higher than has been previously reported for any organism. The enzyme has an apparent Km of 1.7 × 10-5 M and low activity towards L-glutamine when assayed at pH 8.5.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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