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. 1974 Feb;27(2):333–336. doi: 10.1128/am.27.2.333-336.1974

Evaluation of Type A Botulinal Toxin Assays that Use Antitoxin to Crystalline Toxin

H Sugiyama 1,2, I Ohishi 1,2,1, B R Dasgupta 1,2
PMCID: PMC380030  PMID: 4856714

Abstract

The type A botulinal toxin assay by the reverse passive hemagglutination procedure which uses antitoxin to crystalline toxin was examined for specificity. The analysis was based on the fact that crystalline type A toxin is a complex of neurotoxic protein (Aα) and a nontoxic protein (Aβ). By using these components, obtained in essentially pure forms, it was shown that the antitoxin to crystalline toxin has a significantly higher titer to Aβ than to Aα. When Formalin-treated red blood cells were sensitized with this antitoxin, the antibodies coupled to the cells were, for practical results, only anti-Aβ. When the suspension is reacted with dilutions of type A toxic solutions, the limiting dilutions are determined by Aβ and not by the neurotoxin, which should be the determinant if the assay is to measure toxicity. These observations may be pertinent to the development of serological assays for other botulinal toxin types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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