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. 1996 Oct 29;93(22):12406–12411. doi: 10.1073/pnas.93.22.12406

Developmental abnormalities and epimutations associated with DNA hypomethylation mutations.

T Kakutani 1, J A Jeddeloh 1, S K Flowers 1, K Munakata 1, E J Richards 1
PMCID: PMC38004  PMID: 8901594

Abstract

A number of aberrant morphological phenotypes were noted during propagation of the Arabidopsis thaliana DNA hypomethylation mutant, ddm1, by repeated self-pollination. Onset of a spectrum of morphological abnormalities, including defects in leaf structure, flowering time, and flower structure, was strictly associated with the ddm1 mutations. The morphological phenotypes arose at a high frequency in selfed ddm1 mutant lines and some phenotypes became progressively more severe in advancing generations. The transmission of two common morphological trait syndromes in genetic crosses demonstrated that the phenotypes are caused by heritable lesions that develop in ddm1 mutant backgrounds. Loss of cytosine methylation in specific genomic sequences during the selfing regime was noted in the ddm1 mutants. Potential mechanisms for formation of the lesions underlying the morphological abnormalities are discussed.

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