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Effect of Pam3CSK4 on interferon-γ (IFN-γ) -induced inducible nitric oxide synthase (iNOS) protein and mRNA expression. END-D cells were pre-treated with or without Pam3CSK4 at 10 μg/ml for 1 hr and then stimulated with IFN-γ at 100 ng/ml for 24 hr (a) or 6 hr (b). The expression of iNOS protein (a) and mRNA (b) was determined by immunoblotting and RT-PCR, respectively. A typical experiment of three independent experiments is shown.
