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. 1974 Mar;27(3):480–483. doi: 10.1128/am.27.3.480-483.1974

Tryptic Soy Bile-Kanamycin Test for the Identification of Bacteroides fragilis

Valerie Vargo 1, Myroslawa Korzeniowski 1, Earle H Spaulding 1
PMCID: PMC380069  PMID: 4596383

Abstract

A simple and practical test for the identification of Bacteroides fragilis is described. It utilizes two well-known properties of this species, i.e., stimulation of growth by bile and resistance to kanamycin. The test media are a tryptic-soy bile agar plate and a supplemented blood agar plate on which a kanamycin 1,000-μg/ml disk is placed. Incubation is for 24 h at 37 C in GasPak. The results of screening 190 strains, mostly clinical isolates, indicate that B. fragilis can be easily and reliably distinguished from other Bacteroides and from Fusobacterium species by its growth on tryptic-soy bile agar and resistance to kanamycin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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