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. 1974 Mar;27(3):527–530. doi: 10.1128/am.27.3.527-530.1974

Enumeration of Fecal Clostridium perfringens Spores in Egg Yolk-Free Tryptose-Sulfite-Cycloserine Agar

A H W Hauschild 1, R Hilsheimer 1, D W Griffith 1
PMCID: PMC380078  PMID: 4363369

Abstract

The Shahidi-Ferguson perfringens, tryptose-sulfite-cycloserine (TSC), and egg yolk-free TSC agars have been tested for their suitability to enumerate fecal spores of Clostridium perfringens. When these spores comprised at least 20% of the total anaerobe spores, equally accurate counts were obtained in the three media. With lower ratios of C. perfringens spores, the most accurate counts were obtained in egg yolk-free TSC agar. The median C. perfringens spore count of 60 normal fecal specimens was log 3.4/g. A nonmotile, sulfite- and nitrate-reducing Clostridium, not identifiable with any known clostridial species, was isolated from 14 out of 60 fecal specimans. It was not differentiated from C. perfringens in the nitrite motility test, but could be distinguished by its inability to liquefy gelatin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANGELOTTI R., HALL H. E., FOTER M. J., LEWIS K. H. Quantitation of Clostridium perfringens in foods. Appl Microbiol. 1962 May;10:193–199. doi: 10.1128/am.10.3.193-199.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akama K., Otani S. Clostridium perfringens as the flora in the intestine of healthy persons. Jpn J Med Sci Biol. 1970 Jun;23(3):161–175. doi: 10.7883/yoken1952.23.161. [DOI] [PubMed] [Google Scholar]
  3. Cassier M., Sebald M. Germination lysozyme-dépendante des spores de Clostridium perfringens ATCC 3624 après traitement thermique. Ann Inst Pasteur (Paris) 1969 Sep;117(3):312–324. [PubMed] [Google Scholar]
  4. Duncan C. L., Labbe R. G., Reich R. R. Germination of heat- and alkali-altered spores of Clostridium perfringens type A by lysozyme and an initiation protein. J Bacteriol. 1972 Feb;109(2):550–559. doi: 10.1128/jb.109.2.550-559.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOBBS B. C., SMITH M. E., OAKLEY C. L., WARRACK G. H., CRUICKSHANK J. C. Clostridium welchii food poisoning. J Hyg (Lond) 1953 Mar;51(1):75–101. doi: 10.1017/s0022172400015515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall H. E., Hauser G. H. Examination of Feces from Food Handlers for Salmonellae, Shigellae, Enteropathogenic Escherichia coli, and Clostridium perfringens. Appl Microbiol. 1966 Nov;14(6):928–933. doi: 10.1128/am.14.6.928-933.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harmon S. M., Kautter D. A., Peeler J. T. Improved medium for enumeration of Clostridium perfringens. Appl Microbiol. 1971 Oct;22(4):688–692. doi: 10.1128/am.22.4.688-692.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hauschild A. H., Hilsheimer R. Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulfite-cycloserine agar. Appl Microbiol. 1974 Mar;27(3):521–526. doi: 10.1128/am.27.3.521-526.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hauschild A. H., Hilsheimer R. Evaluation and modifications of media for enumeration of Clostridium perfringens. Appl Microbiol. 1974 Jan;27(1):78–82. doi: 10.1128/am.27.1.78-82.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sebald M., Ionesco H. Germination lzP-dépendante des spores de Clostridium botulinum type. C R Acad Sci Hebd Seances Acad Sci D. 1972 Nov 6;275(19):2175–2177. [PubMed] [Google Scholar]
  11. Shahidi S. A., Ferguson A. R. New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens. Appl Microbiol. 1971 Mar;21(3):500–506. doi: 10.1128/am.21.3.500-506.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sutton R. G. Distribution of heat-resistant Clostridium welchii in a rural area of Australia. J Hyg (Lond) 1966 Mar;64(1):65–74. doi: 10.1017/s0022172400040341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sutton R. G. Enumeration of Clostridium welchii in the faeces of varying sections of the human population. J Hyg (Lond) 1966 Sep;64(3):367–374. doi: 10.1017/s0022172400040651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sutton R. G., Hobbs B. C. Food poisoning caused by heat-sensitive Clostridium welchii. A report of five recent outbreaks. J Hyg (Lond) 1968 Mar;66(1):135–146. doi: 10.1017/s0022172400041000. [DOI] [PMC free article] [PubMed] [Google Scholar]

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